Nucleic acid and protein composition and its production method and application in immune regulation

A protein and composition technology, applied to nucleic acid substances and protein compositions and their production and application in immune regulation, can solve problems such as suppressing organ failure, achieve suppressing T cell immune responses, overcome nonspecific, Ease of purification

Inactive Publication Date: 2005-01-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The immunosuppressants and methods commonly used clinically have the following aspects: Utilize chemicals such as Prograf (FK506), cyclosporine A (CsA), CellCept (MMF), azathioprine (Aza) , prednisone (Pred), early-based prednisolone (MP); use antibodies such as: anti-lymphocyte globulin (ALG), anti-CD4 monoclonal antibody (OKT4); but the above immunosuppressants have their toxicity Side effects, if used improperly, on the one hand, it can cause various complications due to excessive suppression of the body's immune reactivity, and on the other hand, it can also lead to the failure of suppressed organ function due to its own toxic side effects

Method used

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  • Nucleic acid and protein composition and its production method and application in immune regulation
  • Nucleic acid and protein composition and its production method and application in immune regulation
  • Nucleic acid and protein composition and its production method and application in immune regulation

Examples

Experimental program
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Effect test

Embodiment 1

[0051] Preparation of Example 1 DNA

[0052] One method is to grind the animal tissue at low temperature, remove the protein in a phenol-chloroform solution, and separate the double-stranded DNA by ethanol precipitation.

[0053] Another method is to extract DNA from plant tissue by CTAB method, remove protein in phenol-chloroform solution, and separate double-stranded DNA by ethanol precipitation.

[0054] Another method is to extract plasmid DNA from Escherichia coli, remove protein in phenol-chloroform solution, and separate double-stranded DNA by ethanol precipitation.

[0055] The detailed description of the above extraction methods and techniques can be edited by "Molecular Cloning" (Second Edition 1998, Cold Spring Harbor Laboratory Press, New York) and Li Chaolong etc. in Sambrook et al., "Biochemistry and Molecular Biology Experimental Technology" Zhejiang University Publisher search.

Embodiment 2

[0056] Embodiment 2: the preparation of protein and polypeptide

[0057] Proteins and peptides can be synthesized by standard automatic peptide synthesizers (such as ABI, 433A, etc.), according to the method used by the instrument manufacturer; or from animal tissues and cells, plant tissues and cells or microorganisms, according to conventional protein chemistry methods For extraction, it can also be extracted from genetically engineered expression bacteria or cells. These polypeptide extraction methods are well known and described in detail in "Protein Purification Protocols" by Doonan (1996, Humana Press, NJ).

Embodiment 3

[0058] Embodiment 3: preparation and inactivation of pathogen

[0059] Viruses, bacteria, mycoplasma, parasites, and allergens that are isolated and produced by pathogens are inactivated by known inactivation methods and reagents such as: formaldehyde or formalin, β-propiolactone, N-acetylethyleneimine, and dioxin Ethylene imine, etc., obtained by inactivating non-infectious pathogens, and then separated and purified.

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Abstract

A composition of nucleic acid and protein or polypeptide or deactivated pathogen or deactivated vaccine containing mineral oil is prepared through proportional mixing. It can be used to effectively suppress the immunoresponse power of specific cells with low by-effect.

Description

1. Technical field [0001] The present invention relates to a nucleic acid substance and protein composition and its production method and its application in the preparation of immune preparations, especially the nucleic acid and protein composition and its composition produced by mixing DNA and protein or inactivated microorganisms Methods of production and use in immunomodulation. 2. Background technology [0002] The body's immune system is always in a complex regulatory process. Immune regulation refers to the interaction between cells in the immune system, between immune cells and immune molecules, and between the immune system and other systems in the process of immune response, forming a network structure that coordinates and restricts each other to maintain the immune response. Appropriate strength, so as to ensure the stability of the internal environment of the body. After the invasion of foreign pathogens, the immune system can activate the required immune respon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K48/00
CPCA61K2039/57A61K48/00A61K39/00A61P1/04A61P3/10A61P5/14A61P5/38A61P7/06A61P9/00A61P11/00A61P11/02A61P11/06A61P13/12A61P17/00A61P17/02A61P19/02A61P21/04A61P25/00A61P29/00A61P31/04A61P31/12A61P33/00A61P35/00A61P37/02A61P37/06A61P37/08
Inventor 王宾俞庆龄
Owner CHINA AGRI UNIV
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