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Preparation process of burduck protoglucoside of anti virus and antiptmour natural medicine

A technology of arctigenin and natural medicine, which can be applied in the directions of anti-tumor drugs, anti-viral agents, drug combinations, etc., and can solve problems such as unsatisfactory

Inactive Publication Date: 2005-01-05
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, water is not ideal as a solvent for the extraction of arctigenin and arctiin

Method used

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  • Preparation process of burduck protoglucoside of anti virus and antiptmour natural medicine
  • Preparation process of burduck protoglucoside of anti virus and antiptmour natural medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Extraction and enzymatic hydrolysis of arctiin crude product

[0024] Grind the burdock fruit medicinal material, add 6-15 times the amount of solvent gasoline or petroleum ether (boiling point range 60°C-90°C), cold soak or reflux extraction for 3 times, and filter. Take the powder and evaporate the solvent. Add 6-15 times the amount of 75% medicinal alcohol, cold soak (percolation) or ultrasonic extraction at room temperature, or heat reflux extraction. Filtrate, combine the filtrates, recover the solvent under reduced pressure, and dry the residue under vacuum to obtain the crude product of arctiin.

[0025] Arctigenin was produced by snail enzymatic hydrolysis of arctiin. Orthogonal design method was used to investigate the optimal conditions of enzymatic hydrolysis. Taking substrate / enzyme mass ratio, reaction time, reaction medium, and solvent pH as four investigation factors, and taking the yield of arctigenin as an index, the influence of each facto...

Embodiment 2

[0030] The enzymatic hydrolysis yield determination of embodiment 2 arctigenin

[0031] 1. Materials and instruments

[0032] The pure product of arctiin is self-made, it is a kind of white powder, [α] D -47° (c1.8, EtOH). Thin-layer chromatography (TLC) showed a single spot, and high-performance liquid chromatography (HPLC) showed a purity of 99%. Its TLC behavior and IR, MS and other spectra are consistent with arctiin reference substance. Helicase was purchased from Shanghai Yuanju Biotechnology Co., Ltd. (batch number 011129). Silicone GF 254 The TLC plate is a product of Qingdao Ocean Chemical Factory. The instruments used also include: HZQ-C constant temperature oscillator (Harbin Donglian Electronic Technology Development Co., Ltd.), Dionex high performance liquid chromatography, P680 infusion pump, ASI-100 automatic sampler, PDA-100 photodiode array detection instrument, Chromeleon chromatographic workstation, PHS-25 digital pH meter (Shanghai Precision Scientifi...

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Abstract

The invention relates to a method of preparing antivirus and antitumor natural medicine arctigenin, and its characteristic: crushing the Arctium lappaL., using organic solvent to degrease, and obtaining a crude arctiin, then using snailase to make fermentation and enzymolysis the on the crude arctiin in an enzyme reaction system, and finally terminating the enzymolysis reaction by alcohol-containing water-soluble organic solvent and extracting enzymolysis products, then separating the enzymolysis products by using silica gel column chromatography and solvent recrystallization, purifying and obtaining the pure arctigenin; the yield of the method is increased by at last over 20 times as compared with that of separating natural-existing arctigenin in plants like Arctium lappaL.

Description

technical field [0001] The invention relates to a method for preparing medicine, in particular to a new method for preparing an antiviral and antitumor active compound arctigenin (arctigenin, English name Arctigenin). The method of the present invention refers to the method of producing arctigenin from arctiin through enzymatic conversion combined with chromatographic separation. [0002] The advancement and practical value of the drug manufacturing method of the present invention lie in that its yield is at least 20 times higher than that of arctigenin isolated from plants such as burdock seeds. The purpose of low-cost industrialized production of arctigenin can be achieved, and the method is economical and practical. Background technique [0003] Arctigenin is a natural organic compound with antiviral, antitumor and immunomodulatory effects. It can be used to treat influenza, common cold and AIDS, or treat tumor diseases such as lung cancer and leukemia. [0004] Burdoc...

Claims

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Application Information

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IPC IPC(8): A61P31/12A61P35/00C12P17/04
Inventor 胡英杰李国桥符林春樊蕴华
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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