Hemopoietic stem/progenitor cell amplifying culture system

Abstract

The present invention relates to hemopoietic stem/progenitor cell amplifying culture system. The hemopoietic stem/progenitor cell amplifying culture system of the present invention contains marrow endothelial cell conditioned medium. The present invention amplifies on endothelial cell conditioned medium, rather than on cell layer directly, and this can avoid virus pollution and cell pollution caused by direct contact between the hemopoietic stem/progenitor cell and the endothelial cell. The cell amplifying culture system the present invention provides obtains satisfied amplification effect of hemopoietic progenitor cell.

Description

Technical field [0001] The invention relates to an expansion culture system of hematopoietic stem / progenitor cells. Background technique [0002] Hematopoietic stem cell transplantation is an epoch-making technology in the field of hematology. It has become a reliable choice for curing some malignant blood diseases, solid tumors, hereditary and immune diseases. There are three types of hematopoietic stem cell transplantation, bone marrow hematopoietic stem cell transplantation (BMT), peripheral blood hematopoietic stem cell transplantation (PBSCT), and cord blood hematopoietic stem cell transplantation (ICBTR). Because umbilical cord blood has a wide range of sources and is convenient to collect, umbilical cord blood is rich in hematopoietic stem / progenitor cells, the immunity of lymphocytes contained in umbilical cord blood is low, and the incidence and severity of graft-versus-host disease (GVHD) are low. Therefore, umbilical cord blood The basic and applied research and ...

AI Technical Summary

Problems solved by technology

The culture method of endothelial cells has been disclosed in the prior art, but it is time-consuming to establish the stromal cell layer, which is not suitable for the needs of large-scale clinical application

[0004] Although umbilical vein endotheli

Images