Compositions, methods and kits for immunochemical determination of mannan-binding lectin (MBL)

A technology of mannan and kits, applied in the field of components and kits for immunochemical determination of mannan-binding lectin (MBL), able to solve problems of different clinical relevance, etc.

Inactive Publication Date: 2005-07-27
ANTIBODYSHOP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, it is unknown whether low measured MBL levels due to structural abnormalities are of different clinical relevance than low normal MBL levels due to LXPA / LXPA genotypes

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0021] The present invention provides antibodies, methods for screening antibodies, and methods and kits for measuring the concentration of mannan-binding lectin (MBL) in a sample using the antibodies in an in vitro method. Using the antibodies, methods and kits of the invention, the presence of variant forms of MBL can be distinguished from the presence of low concentrations of normal MBL. Furthermore, the total amount of MBL can be measured independently of the oligomeric state of MBL and compared to oligomerized MBL capable of binding mannan.

[0022] The antibodies, methods and kits of the invention are particularly useful for the quantitative analysis and characterization of MBL in samples, including biological fluids, particularly human serum and plasma. When using this assay to analyze the ability of MBL to bind mannan in plasma samples, it is best to anticoagulate the blood sample with heparin rather than using a calcium chelator, such as citric acid or EDTA, because M...

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PUM

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Abstract

Various antibodies with different binding characteristics for MBL A or MBL B bound to mannan or MBL A or MBL B bound to an antibody against MBL are provided. Also provided are methods for selecting antibodies against MBL with different binding characteristics and methods and kits for using these antibodies to measure MBL capable of binding to mannan or oligomerized MBL and abnormal, poorly oligomerized MBL in a sample. The methods and kits are useful in diagnosing increased susceptibility to and exacerbation of infections and autoimmune diseases.

Description

field of invention [0001] Mannan-binding lectin (MBL) can be present in different amounts and in different genetically determined variant forms in different individuals, thereby affecting the susceptibility of those individuals to infectious diseases and the severity of autoimmune diseases. The present invention provides a series of monoclonal antibodies against human mannan-binding lectin (MBL), which can be used to quantify the concentration of this protein, especially in biological fluids, such as human serum or plasma Analysis can also be used to provide structural and functional information about the protein. In one embodiment of the present invention, there are provided components, methods and kits for measuring oligomerized MBL capable of binding to mannan. In another embodiment, there are provided components, methods and kits for measuring total immunoreactive MBL subunits whether or not these subunits polymerize into tetramers, pentamers that characterize MBL with fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53C07K16/18C12P21/08G01N33/577G01N33/68
CPCY10S435/81Y10S435/975G01N33/68C07K16/18Y10S435/96G01N2474/20
Inventor 拉尔斯·奥托·乌特恩塔尔
Owner ANTIBODYSHOP
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