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Compound of substituted nonane oxide-2-ketone, preparation method and application

A kind of compound, branched chain alkyl technology, applied in the field of substituted oxanenonane-2-one compounds and their preparation and use

Inactive Publication Date: 2005-08-31
INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports on natural oxanonan-2-ones

Method used

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  • Compound of substituted nonane oxide-2-ketone, preparation method and application
  • Compound of substituted nonane oxide-2-ketone, preparation method and application
  • Compound of substituted nonane oxide-2-ketone, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1 Fermentative Production and Separation and Purification of Compound I

[0080] Fermentation production

[0081] Toxin-producing bacteria

[0082] The toxin-producing bacteria used in this example to ferment and produce oxanonan-2-one compounds was Streptomyces flaviretus 18522 CGMCC 1022 strain isolated from soil samples in Xishuangbanna and identified through taxonomic research.

[0083] Fermentation culture of toxin-producing bacteria

[0084] According to the conventional method for culturing microorganisms, an appropriate amount of Streptomycesflavoretus 18522 CGMCC 1022 was inoculated onto the solid slant medium of Gaoshi Synthetic No. 1 agar, and cultured in an incubator at 28°C for 7 days.

[0085] Get the slant that has been cultivated for 7 days, pick an appropriate amount of spores and thalline with an inoculation needle, and inoculate into a medium containing 100 milliliters of seed culture solution (substrate composition: soybean powder 0.5%, solub...

Embodiment 2

[0109] Example 2 Tests for Inhibition of Proliferation, Cell Cycle Inhibition, and Apoptosis-Inducing Activity of Cancer Cells

[0110] Experimental samples and experimental methods

[0111] Preparation of the tested sample solution

[0112] The test sample is the pure compound I isolated and refined in Example 1 above. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0113] Subculture of cell lines and cells

[0114] The activity test uses mammalian cancer cell lines such as human chronic myelogenous leukemia K562 cells, human colon cancer HCT-15 cells, human ovarian cancer A2780 cells, human lung cancer A549 cells, human cervical cancer HeLa cells and mouse breast cancer tsFT210 cells. All kinds of cells were fed with RPMI-1640 medium containing 10% FBS at 32°C (tsFT210 cells) or at 37°C (K562 cells, HCT-15 cells, A2780 cells, A549 cells and HeLa cells). Subculture in a carb...

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PUM

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Abstract

A substituted oxyheteroarylnonane-2-one compound used for preparing cell cycle depressant, cell wither inducer and antineoplastic medicine isprepared from streptomyces flavorectus.

Description

Technical field: [0001] The present invention relates to a new substituted oxacyclononan-2-one compound and a method for preparing a substituted oxacyclononan-2-one compound; the present invention also relates to the use of this type of compound in the preparation of cell cycle inhibitors or cell regulators. Death inducers and antitumor drugs. Background technique: [0002] There have been many reports on compounds with anti-tumor activities such as cell cycle inhibition and apoptosis induction in microbial fermentation products and their preparation methods. As in literature C.-B.Cui, etal.; Novel mammalian cell cycle inhibitors, cyclotryprostatinsA-D produced by Aspergillus fumigatus, which inhibit mammaliancell cycle at G2 / M phase: Tetrahedron, Vol.53, No.1, pp.59 -72, 1997 and Hiroyuki Nagata, etc., new regulation substance アセトフタリジン, cell cycle inhibitor ㉤ りよび anti-tumor section, Japanese patent, open license Hiroyuki A), Japanese Patent Application Hei 9-87269, announc...

Claims

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Application Information

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IPC IPC(8): A61K31/365A61P35/00C07D313/00C12P17/08
Inventor 崔承彬韩冰蔡兵
Owner INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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