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Combined process ofr producing animal blood fiber protein

A fibronectin and animal blood technology, applied in the field of biomedicine, can solve the problems of unsuitability for industrial scale production, complex process, high cost, saving chromatography medium and production cycle, increasing the amount of materials, and high equipment utilization. Effect

Active Publication Date: 2005-12-21
郑州德福恩生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation methods reported so far are per unit yield, the process is complicated, the cost is high, and it is not suitable for industrial scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The production method of FN co-production process with 1000 milliliters of animal blood is as follows:

[0021] 1. Collect 1000 ml of fresh animal blood, add 100 ml of 3.8% trisodium citrate anticoagulant, centrifuge for 10 minutes at 5000 rpm, collect blood cells and plasma respectively, and store in an environment below 10 degrees.

[0022] 2. To the obtained plasma, add ammonium sulfate by volume to a saturation of 20%, stir to dissolve and let stand for more than 1 hour. Filter or hang filter, respectively collect fibronectin precipitate and filtrate.

[0023] Dissolve the fibronectin precipitate, put it on the affinity chromatography column, the above affinity chromatography column is gelatin-agarose gel 2B, extract and isolate the FN main peak liquid; then pass through the molecular sieve chromatography column, the described The molecular sieve chromatography column is Sephadex G75 for purification, and the purified solution is concentrated by ultrafiltration, st...

Embodiment 2

[0029] 1. Collect 1000 ml of fresh animal blood, add 142 ml of 3.8% trisodium citrate anticoagulant, centrifuge for 20 minutes at 3000 rpm, and collect blood cells and plasma respectively.

[0030] 2. Add ammonium sulfate to the blood plasma by volume to a saturation of 30%, stir and dissolve, and let it stand for more than 1 hour. Filter or hang filter to collect the precipitate and filtrate respectively. The precipitate is dissolved on an affinity chromatography column, the upper affinity chromatography column is gelatin-agarose 6B, and then passed through a molecular sieve chromatography column, and the molecular sieve chromatography column is Sephadex G200 , for purification, the purified solution was concentrated by ultrafiltration, and freeze-dried to obtain the finished product of FN.

[0031] Ammonium sulfate was added to the filtrate for the second time according to the volume until the degree of homogeneity was 60%, filtered, and the precipitate was collected, desal...

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PUM

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Abstract

This invention has disclosed an animal blood fibre connecting albumens united craft of separating and distilling several kinds of biochemical materials such as FN, SOD, immunoglobulin (Ig) and hemachrome. Gathering the fresh animal's blood, it is anticoagulatory to add trisodium citrate, centrifugate rapidly, collect blood plasma and blood corpuscle separately, store it in the environment under 10 degrees, add ammonium sulphate to the blood plasma until the saturation is about 20 to 30 percent, put above l hour quietly, filter or hang filter, fetch the precipitate to get FN product, it has abundant material source and easy craft, suitable for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a process for co-producing animal blood fibronectin by separating and extracting several biochemical substances such as FN, SOD, immunoglobulin and heme in the same process flow. Background technique [0002] Fibronectin is also known as "fibronectin", "fibronectin", the domestic common name "FN", English Fibronectin. FN is a macromolecular glycoprotein discovered by foreign research in 1974, which has various biological activities and functions. At present, a large number of researches on the application of FN at home and abroad are mainly manifested in two aspects: 1. As a biochemical reagent, it provides the basis for the industrial application of cell culture and bioengineering. 2. Applied to the diagnosis and treatment of various diseases (such as: wound repair and healing, cancer diagnosis and treatment, etc.). The US sigma company has produced bovine bloo...

Claims

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Application Information

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IPC IPC(8): C07K14/78
Inventor 王德亮
Owner 郑州德福恩生物技术有限公司
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