Method for establishing high frequency regenerating system of perennial ryegrass

A technology of high-frequency regeneration and ryegrass, which is applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of poor ability to regenerate plants, difficult cultivation, slow progress of perennial ryegrass, and achieve the goal of obtaining materials Convenience and the effect of increasing the induction rate
CN1732758AInactive Publication Date: 2006-02-15BEIJING FORESTRY UNIVERSITY +2
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
BEIJING FORESTRY UNIVERSITY
Publication Date
2006-02-15
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a method for establishing a high-frequent regenerating system for the perennial ryegrass, providing good receptor material for the work of genetic conversion, comprising: sterilizing the seeds, inducing the embryo callus on the traumatic inducing culture medium by employing the mature embryo as explant, shifting it to the regenerative culture medium after subculture for the regenerative sprout growth, then shifting it to the root-growing culture medium for the root growth and replanting it after getting the complete plant. The draw material of explant is easy and free of seasonal constrain, the sterilizing mode is safe and effective, by regulating the concentration of the hormone and addictive in the traumatic inducing culture medium, growing culture medium and root-growing culture medium, the inductivity of embryo callus is increased effectively, and the callus embryo structure is kept in the course of subculture; the regenerative rate and root-growing rate is very high and the survival rate of replanting is 100%.
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Description

Technical field:

[0001] The invention relates to a method for establishing a high-frequency regeneration system of perennial ryegrass, which belongs to a plant tissue culture method and can be used for the cultivation of receptor materials for gene transformation such as gene gun method and agrobacterium-mediated method. technical background:

[0002] At present, using genetic engineering to breed and improve plant varieties is a convenient and practical way. Successful plant gene transformation firstly depends on a good plant receptor system. Such plant receptor systems for transgenics must have high-frequency, stable regeneration capabilities, and good sensitivity to transformation screening agents. The establishment and improvement of plant tissue culture and regeneration system is the basis and premise of genetic transformation. However, due to the characteristics of gramineous plants, the tissue culture and regeneration systems of many forage grasses and turfgrasses a...

Claims

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