Determination of magnesium ion content from enzyme method and magnesium ion diagnostic reagent kit

A diagnostic kit and magnesium ion technology, applied in the field of medical testing and determination, can solve problems such as affecting use and promotion, and differences in the accuracy of sensitivity test results.
CN1778962AInactive Publication Date: 2006-05-31SUZHOU ANJ BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SUZHOU ANJ BIOTECHNOLOGY CO LTD
Publication Date
2006-05-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention is about the enzyme method of measuring magnesium ion and its diagnosis reagent box. Making use of the peculiarity that magnesium ion in the sample of plasma or serum and so on can activate the activation of glycerokinase, and producing glycerol-3-phosphoryl by reacting glycerol under the existence of adenosine triphosphate, and then causing coupled reaction with glycerol-3-phosphoryl dehydrogenase, hydroperoxide enzyme and aldehyde dehydrogenase and transferring oxidized coenzyme to reduced coenzyme. Testing the ascending range of dominant wave-length 340nm absorbance and finally measuring the content of magnesium ion in the sample. This method has high specificity and would not be contaminated by material of internal and exogenous sources, and the result is precise and accurate. Diving the diagnosis reagent box into double-dose or three-dose can reduces the cross interaction of each element, keeps the stability of the reagent and deposits chronically. Using this method can realize the fast testing in common ultraviolet / visible light analyzer or semiautomatic / automatic analyzer and doesní»t require special or additional apparatus, so the cost is low. Thus, this method can be easily promoted and applied in the whole industry.
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Description

technical field

[0001] The invention relates to a method for enzymatically measuring magnesium ion content in samples such as plasma and serum, and a magnesium ion diagnostic kit prepared by applying the method, which belongs to the technical field of medical inspection and determination. Background technique

[0002] At present, there are many methods for the determination of magnesium ion content in samples such as plasma and serum (referred to as magnesium determination), which can be summarized as follows: colorimetric method, fluorescence method, ion chromatography, ion selective electrode method (ISE), enzymatic method, Atomic absorption spectrophotometry (AAS), isotope dilution mass spectrometry (ID-MS), etc. Among them, the decisive method is ID-MS, followed by AAS. Although the results of these two methods are accurate, the equipment is complex and expensive, and they are not suitable for routine laboratory and automatic analysis.

[0003] Colorimetry is the most w...

Claims

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