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Porcine pancreatic islet cultured with porcine sertoli cells for xenotransplantation

A technology to support cells and islets, applied in the field of diabetes treatment

Inactive Publication Date: 2006-06-21
戴伯塞尔有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] 2. Possible transmission of infectious diseases: This potential issue focuses on the risk of disease transmission from pigs to recipients, and the risk of introducing microorganisms during cell manipulation
[0018] 3. Ethical issues related to xenotransplantation: This includes issues of whether the use of animal tissue for transplantation is ethically acceptable, issues of welfare of donor animals, issues of obtaining informed consent from patients selected for clinical trials and the process impact on them

Method used

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  • Porcine pancreatic islet cultured with porcine sertoli cells for xenotransplantation
  • Porcine pancreatic islet cultured with porcine sertoli cells for xenotransplantation
  • Porcine pancreatic islet cultured with porcine sertoli cells for xenotransplantation

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Embodiment Construction

[0067] The present invention relates to the preparation and use of "aggregates" of Sertoli cells and porcine islets.

[0068] Prior art methods involving the use of Sertoli cells and islets (or other cells) typically involve handling and separating them separately and bringing them together for transplantation.

[0069] Applicants have found that making aggregates and co-culturing at a predetermined ratio of islets to Sertoli cells allows islets to have time to grow and utilize growth factors believed to be derived from Sertoli cells in vitro prior to transplantation. Applicants have discovered that islets are more functional when protected with a layer of supporting cells.

[0070] Ideally, islets and supporting cells are from the same donor. This simplifies virus screening.

[0071] The term "aggregate" as used herein specifically refers to a discontinuous layer of supporting cells on the surface of the native islet structure.

[0072] Rationale for co-transplantation of ...

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Abstract

Aggregates and their method of preparation suitable for implantation into a recipient in order to produce insulin in vivo. The methods involve culturing islet cells isolated from the pancreas of donor piglets with isolated Sertol i cells from the testes of donor piglets. A preferred period of culturing is 5 days and may be followed by a purification procedure.

Description

technical field [0001] The invention relates to the use of porcine pancreatic islet cells for treating diabetes. More particularly but not exclusively, the present invention relates to the use of porcine pancreatic islet cells together with Sertoli cells for treating diabetes by xenotransplantation. Background technique [0002] Background and rationale of porcine islet cell xenotransplantation [0003] Type 1 (insulin-dependent) diabetes mellitus is a common endocrine disorder that causes considerable morbidity and mortality and has serious economic implications for individual patients and healthcare systems. Although life-saving, treatment with insulin often does not provide sufficient control of blood glucose to prevent life-shortening complications of the disease, prompting extensive research on better ways to achieve and maintain normoglycemia . Among the newer therapeutic strategies proposed, transplantation of pancreatic beta islet cells obtained from human beings ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/12A61K35/39C12N5/071
CPCA61K35/12A61K35/39C12N5/0676C12N5/0677C12N2502/246C12N2510/02A61P3/10
Inventor 斯蒂芬·约翰·马丁·斯金纳罗伯特·巴特利特·埃利奥特利维亚·德尔·卡门·埃斯科巴尔·奥雷利亚纳
Owner 戴伯塞尔有限公司
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