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Finger print atlas determination method of Chinese medicinal material tangerine peel water extraction

A technology of fingerprint and determination method, which is applied in the field of determination of fingerprint of water extract of tangerine peel, a traditional Chinese medicinal material, can solve problems such as insufficiency, and achieve the effects of simple method, improved purity, improved column efficiency and service life

Active Publication Date: 2006-09-13
JIANGZHONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are a lot of researches on the pharmacological effects of the polar part of tangerine peel, research shows that the pharmacological effects of the polar part of tangerine peel are very wide, such as: Shen Mingqin, Clinical Pharmacy, 1997, 2, 32 (2), 97~100; Xu Peng , Journal of Jiangxi University of Traditional Chinese Medicine, 1998, 10(4), 172~173: Wang Meimei, etc., Journal of China Pharmaceutical University, 1998, 29(6), 462~464; and to control the efficacy of tangerine peel and its prescription preparations However, it is far from enough to only focus on one or two chemical components. It is necessary to control its material group as a whole to characterize the quality characteristics of Chenpi medicinal materials as a whole. At present, the research that can characterize the overall characteristics of the polar part of Chenpi is still blank.

Method used

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  • Finger print atlas determination method of Chinese medicinal material tangerine peel water extraction
  • Finger print atlas determination method of Chinese medicinal material tangerine peel water extraction
  • Finger print atlas determination method of Chinese medicinal material tangerine peel water extraction

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (a) Preparation of the test solution: Weigh the tangerine peel of the medicinal material through a 24-80 mesh sieve, put it in a flat-bottomed flask, add 8 times the amount of water, heat, reflux for extraction for 3 hours, let cool to 30-50°C, vacuum filter, and Add 6 times the amount of water to the slag, extract for 2 hours, operate in the same way, combine the filtrates, and measure the volume. Measure 5g of the filtrate relative to the crude medicinal material, pass through the AB-8 resin column, wash with 3 to 4 times the column volume of water, then wash with 3 to 4 times the column volume of 15% ethanol or 15% methanol, discard and elute Finally, elute with 90% ethanol or 90% methanol of 3 to 4 times the column volume, collect the 90% ethanol or 90% methanol eluate, evaporate the ethanol or methanol in a water bath, transfer it to a 125ml measuring bottle, add pure water To the scale, it is the test sample;

[0048] (b) Chromatographic conditions: detection wav...

Embodiment 2

[0051] (a) Preparation of the test solution: Weigh the 24-80 mesh sieved dried tangerine peel, put it in a flat-bottomed flask, add 80 times the amount of water, heat, reflux and extract for 0.5h, let cool to 30-50°C, vacuum filter, Add 60 times the amount of water to the dregs, extract for 0.25h, operate in the same way, combine the filtrates, and measure the volume. Measure 0.25g of the filtrate relative to the raw medicinal material, pass through an AB-8 resin column, wash with 3 to 4 times the column volume of water, then wash with 3 to 4 times the column volume of 5% ethanol or 5% methanol, discard the washed Remove the liquid, and finally elute with 40% ethanol or 40% methanol of 3 to 4 times the column volume, collect the 40% ethanol or 40% methanol eluate, evaporate the ethanol or methanol in a water bath, transfer to a 2ml measuring bottle, and add purification When the water reaches the mark, it is the test sample;

[0052] (b) Chromatographic conditions: detection ...

Embodiment 3

[0055] (a) Preparation of the test solution: Weigh the 24-80 mesh sieved dried tangerine peel, put it in a flat-bottomed flask, add 20 times the amount of water, heat, reflux and extract for 2.5 hours, let cool to 30-50°C, vacuum filter, Add 15 times the amount of water to the dregs, extract for 1.5 hours, operate in the same way, combine the filtrates, and measure the volume. Measure 3g of the filtrate relative to the raw medicinal material, pass through the AB-8 resin column, wash with 3 to 4 times the column volume of water, then wash with 3 to 4 times the column volume of 14% ethanol or 14% methanol, discard and elute Finally, elute with 3 to 4 times column volume of 85% ethanol or 85% methanol, collect the 85% ethanol or 85% methanol eluate, evaporate the ethanol or methanol in a water bath, transfer to a 5ml measuring bottle, add pure water To the scale, it is the test sample;

[0056] (b) Chromatographic conditions: detection wavelength: 340 nm; column temperature: 30°...

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PUM

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Abstract

A fingerprint assay method for detecting the aquatic extract of tangerine peel to evaluate the quality of tangerine peel includes such steps as adding the tangerine peel in water, reflux extracting, filtering, adsorbing by macro-reticular resin column, water washing, washing with ethanol or methanol, eluting with ethanol or methanol, collecting eluting liquid, evaporating off ethanol or methanol, loading in measuring glass, adding water, and efficient liquid-phase chromatography to obtain fingerprint.

Description

technical field [0001] The invention relates to a method for establishing the fingerprint of dried tangerine peel, a Chinese medicinal material, in particular to the establishment of the HPLC fingerprint of the water-soluble active ingredient in the dried orange peel of the traditional Chinese medicinal material, and also relates to the standard fingerprint of the dried orange peel medicinal material obtained by the method. Background technique [0002] Chenpi (Pericarpium CitriReticulatae) is the dry mature peel of Rutaceae plant orange (Citrus reticulata Blanco) and its cultivars. It has the functions of regulating qi and strengthening the spleen, drying dampness and reducing phlegm. embolism. The tangerine peel crude drug contains volatile oil, nobiletin, synephrine and flavonoids; the flavonoids include: hesperidin, neonobiletin, citrusin and other components, of which hesperidin is the main component. At present, to the quality appraisal of d...

Claims

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Application Information

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IPC IPC(8): A61K36/752G01N30/02
Inventor 付玉梅李诒光伍振峰
Owner JIANGZHONG PHARMA
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