Method of purifying abamectin kind medicine and its immune affinity chromatographic column

An abamectin, immunophilic technology, applied in chemical instruments and methods, biological testing, other chemical processes, etc., can solve the problems of non-commercialized IAC column sales, waste of organic solvents, and cumbersome processing, and reduce Analysis of cost and environmental pollution, improvement of analysis quality, good effect of purification

Inactive Publication Date: 2006-09-13
CHINA AGRI UNIV
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  • Summary
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Problems solved by technology

The pretreatment of these methods utilizes liquid-liquid distribution and conventional SPE column purification and separation, all of which have disadvantages such as cumbersome treatment process, poor purification effect, waste of organic solvents, and long time required to varying degrees.
Immunoaffinity technology is a new technology that has been applied in the field of analysis in the 1990s, but there is no report on the use of immunoaffinity columns to purify abamectin, doramectin, ebemectin and ivermectin in the matrix. No commercial IAC column for sale

Method used

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  • Method of purifying abamectin kind medicine and its immune affinity chromatographic column
  • Method of purifying abamectin kind medicine and its immune affinity chromatographic column
  • Method of purifying abamectin kind medicine and its immune affinity chromatographic column

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Embodiment 1

[0027]Embodiment 1, the preparation of the immunochromatographic column of purifying Abamectin and / or Ivermectin and / or Ebamectin and / or Doramectin

[0028] 1. Preparation of Abamectin Rabbit Polyclonal Antiserum

[0029] The synthesis of abamectin hapten is divided into three steps:

[0030] (1) 5-o-t-BuMe 2 Synthesis of Si-AVM-4″-OH: Put 1.0g of avermectin in a 25mL three-necked chicken heart bottle, dissolve 6mL of N,N-dimethylamide (DMF), add 0.47g of imidazole, and mix. Under mechanical stirring , 0.52 g of t-BuMe diluted in 2 mL of DMF 2 SiCl (tert-butyldimethylchlorosilane) was added dropwise and reacted at 30°C for 2h. 100 mL of ethyl acetate (EtoAc) was added to the reaction liquid and mixed. The mixture was washed 3 times with 50 mL of water. Separate the EtoAc layer, MgSO 4 Dry and concentrate under reduced pressure to obtain a yellowish viscous substance. The yellowish viscous material was washed with CH 2 Cl 2 Dissolved, the product was separated by silic...

Embodiment 2

[0061] Example 2, Preparation of a kit containing an immunochromatographic column coupled to a rabbit polyclonal antibody or a mouse monoclonal antibody and its purification effect on avermectins

[0062] 1. Preparation of the test kit of Abamectin

[0063] The kit mainly consists of a box body, an immunochromatographic column (IAC column), abamectin standard solution, ivermectin standard solution, doramectin standard solution, ebimectin standard solution, washing solution I, washing solution II, washing solution III, eluting solution, preservation solution, and sponge bracket are composed of holes and grooves on the sponge bracket. The groove of the sponge bracket is filled with abamectin standard solution, ivermectin standard solution, doramectin standard solution, ebimectin standard solution, washing solution I, washing solution II, washing solution III, The eluent, the reagent bottle of the preservation solution, and the hole of the sponge bracket are equipped with IAC co...

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Abstract

A method for purifying abamectin medicines by the special immunoaffinity chromatographic column carrying immunoaffinity adsorbent is disclosed. Said adsorbent is composed of solid carrier and its coupled abamectin polyclonal or monoclonal antibody. It features that the chromatography is used to measure the contents of abamectin, having high correctness.

Description

technical field [0001] The present invention relates to a kind of purifying avermectins (Aermectins, AVMs), comprise avermectin (Aermectin, AVM), doramectin (Doramectin, DOR), ebimectin (Eprinomectin, EP) and i The method of Ivermectin (IVM) and its special immunoaffinity column. Background technique [0002] With the development of life sciences, people have become more and more interested in the substances and their changes in living organisms, and the analysis of biological samples has become a necessary means to explore and discover the mysteries of life. Due to the complex components of biological samples, the concentration of analytes is low, and most of the sample volumes are small, which puts forward higher requirements for the selectivity and sensitivity of the analytical method. Immunoaffinity chromatography (IAC, immunoaffinity chromatography) is an analytical method that combines an immune response with a chromatographic analysis method. Its high selectivity an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/281G01N30/00G01N33/53
Inventor 沈建忠史为民何继红何方洋侯晓林吴聪明
Owner CHINA AGRI UNIV
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