Method for transferring Sichuan pickle using TuMV Hc-Pro resistant gene

A turnip mosaic virus and gene transformation technology, which is applied in the field of bioengineering, can solve the problems such as the insignificant effect of CP gene, and achieve the effect of avoiding chimeric transgenic plants and improving transformation efficiency.
CN1839680AInactive Publication Date: 2006-10-04ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2006-10-04
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

This invention discloses a method for obtaining turnip mosaic antiviral preserved szechuan pickle by means of Hc-Pro gene inversion, the method takes twice subcultured cotyledon inducted wound-healing tissue as inversion receptor, and co-culture the tissue with meloidogynosis peasant bacillus culture liquid with Hc-Pro gene, then continuously sieve the inversed wound-healing tissue on antibiotics added medium, last do differentiation and replantation. Compared with current technics, the beneficial effects of this invention are:Hc-Pro gene inversion is process on cell, and this can improve inversion efficiency and inheritance stability of the gene transferred plant, and can obtain Hc-Pro gene taking plant instead of chimera gene transferred plant.
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Description

technical field

[0001] The invention relates to the field of bioengineering, in particular to a method for transforming pickled mustard with anti-turnip mosaic virus Hc-Pro gene. Background technique

[0002] Mustard mustard is one of the important vegetable crops in my country. It is sold well all over the country and exported to all over the world. Virus disease is the main disease of mustard mustard, and it is relatively common. Viral diseases are caused by turnip mosaic virus and cucumber mosaic virus, among which turnip mosaic virus (TuMV) is one of the most prominent problems. In popular years in history, the average rate of diseased plants in large areas was more than 20%, and in seriously diseased areas and fields, it was as high as more than 50%, and even the whole field had no harvest. In recent years, it has occurred year after year in the main pickled mustard producing areas of our country, and the trend is getting worse year by year. Therefore, the voice of a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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