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Method for transferring Sichuan pickle using TuMV Hc-Pro resistant gene

A turnip mosaic virus and gene transformation technology, which is applied in the field of bioengineering, can solve the problems such as the insignificant effect of CP gene, and achieve the effect of avoiding chimeric transgenic plants and improving transformation efficiency.

Inactive Publication Date: 2006-10-04
ZHEJIANG UNIV
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Problems solved by technology

However, the effect of CP gene on some crops is not obvious, even without

Method used

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  • Method for transferring Sichuan pickle using TuMV Hc-Pro resistant gene
  • Method for transferring Sichuan pickle using TuMV Hc-Pro resistant gene
  • Method for transferring Sichuan pickle using TuMV Hc-Pro resistant gene

Examples

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Embodiment 1

[0025] In this embodiment, the male sterile line and maintainer line of pickled mustard that have been self-bred for many years are used as materials, and the cotyledons of sterile seedlings that are 7-8 days old are used for callus induction.

[0026] The strain of Agrobacterium tumefaciens used in this example is EHA105 (Plh-IR) (purchased from Institute of Biology, Zhejiang University). Plasmid contains tandem Hc-Pro gene and herbicide-resistant Bar gene (such as figure 1 ).

[0027] Induction of callus: Rinse the self-fertilized pickled mustard seeds (Zhejiang University Institute of Vegetables) with tap water for 1 min after washing with tap water, rinse with distilled water for 3 times and stir in 1% NaClO aqueous solution for 15 min. Rinse with sterile water 4-5 times, and sow the seeds on the MS+0.1mg / L BA seeding medium on the ultra-clean workbench. After germination in a dark place at 23-25°C, culture under continuous light for 16h / d at the same temperature. Cut t...

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Abstract

This invention discloses a method for obtaining turnip mosaic antiviral preserved szechuan pickle by means of Hc-Pro gene inversion, the method takes twice subcultured cotyledon inducted wound-healing tissue as inversion receptor, and co-culture the tissue with meloidogynosis peasant bacillus culture liquid with Hc-Pro gene, then continuously sieve the inversed wound-healing tissue on antibiotics added medium, last do differentiation and replantation. Compared with current technics, the beneficial effects of this invention are:Hc-Pro gene inversion is process on cell, and this can improve inversion efficiency and inheritance stability of the gene transferred plant, and can obtain Hc-Pro gene taking plant instead of chimera gene transferred plant.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for transforming pickled mustard with anti-turnip mosaic virus Hc-Pro gene. Background technique [0002] Mustard mustard is one of the important vegetable crops in my country. It is sold well all over the country and exported to all over the world. Virus disease is the main disease of mustard mustard, and it is relatively common. Viral diseases are caused by turnip mosaic virus and cucumber mosaic virus, among which turnip mosaic virus (TuMV) is one of the most prominent problems. In popular years in history, the average rate of diseased plants in large areas was more than 20%, and in seriously diseased areas and fields, it was as high as more than 50%, and even the whole field had no harvest. In recent years, it has occurred year after year in the main pickled mustard producing areas of our country, and the trend is getting worse year by year. Therefore, the voice of a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01H4/00C12N5/04A01C1/00
Inventor 陈利萍
Owner ZHEJIANG UNIV
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