Hepatitis B and C and multiple tumor marker protein chip inspecting reagent unit

A technology for hepatitis C and B and C, which is applied in the field of in vitro clinical testing and biochips, can solve the problems of laborious, expensive, unfavorable routine physical examination, popularization of civilians, etc.

Inactive Publication Date: 2006-11-01
穆海东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Since the traditional serum detection method (the ELISA method is the mainstream in the market now) can only detect one indicator at a time, it is time-consuming, laborious and expensive...

Method used

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  • Hepatitis B and C and multiple tumor marker protein chip inspecting reagent unit
  • Hepatitis B and C and multiple tumor marker protein chip inspecting reagent unit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of detection reaction plate

[0037] Take a 48-well plate, set 42 sample wells and 6 standard wells. A solid phase support (NC membrane or PDVF membrane) is placed at the bottom of each reaction well, and the solid phase support is coated with anti-HBsAg, HCVAg, anti-AFP, anti-CEA, anti-CA199, anti-CA125, anti-PSA and anti- Protein microarrays of eight antigens or antibodies such as fPSA ( figure 1 ).

Embodiment 2

[0039] kit preparation

[0040] Prepare a test kit containing eight indicators of hepatitis B and C and various tumor diseases for integrated detection. It contains one reaction well plate (24-48 people) of Example 1, one bottle of washing concentrate, and one enzyme-labeled working solution. One bottle of detection solution A, one bottle of detection solution B, and a standard bottle.

[0041] Washing Concentrate, Enzyme Label Working Solution, Detection Solution A, Detection Solution B

Embodiment 3

[0043] Integrated detection of eight indicators of hepatitis B and C and various tumor diseases

[0044] Test each blood sample according to the following method:

[0045] 1. Add test blood or standard substance to the corresponding reaction well, 75ul / well.

[0046] 2. Add enzyme-labeled working solution, 75ul / well.

[0047] 3. Incubate and shake at 37°C for 60 minutes.

[0048] 4. Wash 4-6 times with lotion, 2 minutes each time, vibrate.

[0049] 5. Add detection solution 20ul / well, so that the detection solution is evenly distributed at the bottom of the well.

[0050] 6. CCD detection, exposure for 30-60 seconds depending on the strength of the luminescent signal.

[0051] Result judgment:

[0052] According to the CutOff value of different indicators, refer to the gradient curve of the standard to determine the negative or positive of the sample serum.

[0053] At the same time, the serum samples tested were routinely tested by conventional methods. The result show...

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Abstract

A detection kit of marker substance protein chip for hepatitis B and C as well as multiple type of tumor is prepared as having base plate and reaction holes on it set on reaction hole plate; including 12-200 sample holes and 5-8 standard piece holes in said reaction holes as each reaction hole bottom being set with solid phase carrier; enveloping micro lattice of eight antigen or and body as anti-HBsAg, anti-HCVAg, anti-AFP, anti-CEA, anti-CA199, anti-CA125, anti-PSA and anti-fPSA on said carrier for accurately diagnose said disease in multiple man-share.

Description

technical field [0001] The invention belongs to the field of in vitro clinical testing and biochip technology. Specifically, it is an eight-item index (HBsAg, HCVAb, AFP, CEA, CA199) suitable for the diagnosis, prediction and prognosis of hepatitis B and C and various tumor diseases. , CA125, PSA and fPSA) reaction plates and kits for integrated detection Background technique [0002] Hepatitis is a serious disease that is widely prevalent in the world and seriously endangers human health and even life. Among them, hepatitis B and C are the most prevalent and harmful to humans. my country is the country with the most severe hepatitis B virus infection in the world. About 120 million people are carriers of hepatitis B virus, accounting for one-third of the world, of which 30 million are chronic hepatitis, liver cirrhosis, and primary liver cancer, and there are 300,000 people every year. Died of chronic liver disease, about 130,000 of them were primary liver cancer patients. ...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/52G01N33/544
Inventor 穆海东
Owner 穆海东
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