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A method for selecting cadium toxicity-resistant genotype barley

A screening method and genotype technology, which can be used in plant genetic improvement, botanical equipment and methods, seed and rhizome treatment, etc., and can solve problems such as heavy metal stress tolerance that has not yet been developed

Inactive Publication Date: 2006-12-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no research has been carried out on the application of chlorophyll meter to diagnose the tolerance of heavy metal stress in crops

Method used

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  • A method for selecting cadium toxicity-resistant genotype barley
  • A method for selecting cadium toxicity-resistant genotype barley

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Choose 106 parts of different types and growth habit barley germplasm (genotypes), 150 selected seeds of each genotype, and the concentration by weight is 2% Ca(ClO) 2 After sterilizing for 20 minutes, rinse with distilled water 7 times, germinate in the dark on a sand bed in an artificial climate box at 25°C for 3 days, grow for 7 days under 15 hours of light at 20°C / 9 hours of darkness at 18°C ​​after germination, and 10 days after sowing ( Including 3 days of dark germination and 7 days of growth under 15 hours of light at 20°C / 9 hours of darkness at 18°C ​​after germination), at the 2-leaf stage, the wheat seedlings with the same growth were selected and moved to the greenhouse for pre-cultivation in cadmium-free medium for 5 days, and then cadmium-free Treatment: Set 0 (control, CK), 20μM Cd two kinds of treatment, Cd with CdCl 2 form supply. Split-plot design, cadmium treatment as the main plot, variety as the sub-plot, repeated 3 times.

[0027] The cadmium-fre...

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Abstract

The invention disclose a kind of screen method for barley re-cadmium-poison genotype, comprise select seed, disinfect, culture seedling, transplant, pre-culture, cadmium coerion treatment, re-cadmium-poison evaluation; cadmium treat 20 days, detect value of the chlorophyll device for the middle of the functional leave; the decline ratio(%)= 100* (20Umcd treatment detection value - concrete with detection value) / concrete with detection value ; the integration score of each breed= value of the chlorophyll device declined ratio*0.5+ root volume declined ratio*0.1+root number declined ratio*0.1+ dry weight of underground declined ratio*0.1+stalk high declined ratio*0.1. The invention constructor the effect index and method for screening barley re-cadmium-poison genotype-- value of the chlorophyll device, and assistant other modality and physiologic index to analysis and detect.

Description

technical field [0001] The invention belongs to the variety screening technology in the field of agriculture, in particular to a screening method for germplasm resistant to heavy metal cadmium poisoning, and is a rapid screening method for cadmium-resistant / tolerant barley breeding materials. Background technique [0002] Cadmium (Cd) is a heavy metal that is toxic to humans, animals and plants. Due to various reasons such as the discharge of industrial "three wastes", the increase in the amount of pesticides and fertilizers, and the poor disposal of solid waste, the cadmium content in farmland soil is increasing, and the cadmium pollution in the soil-plant-food system is becoming more and more serious, which makes the safety of agricultural products. being threatened. According to reports, rice cadmium pollution is the main problem of heavy metal pollution in Japan; the determination and analysis of edible cereal grains in Japan, South Korea and different regions of my cou...

Claims

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Application Information

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IPC IPC(8): A01H1/04A01C1/00A01G1/00
Inventor 邬飞波陈飞董静汪芳张国平
Owner ZHEJIANG UNIV