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A method for selecting cadium toxicity-resistant genotype barley

A screening method and genotyping technology, applied in the direction of plant genetic improvement, botany equipment and methods, applications, etc., can solve the problems of heavy metal stress tolerance that have not yet been developed

Inactive Publication Date: 2009-07-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no research has been carried out on the application of chlorophyll meter to diagnose the tolerance of heavy metal stress in crops

Method used

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  • A method for selecting cadium toxicity-resistant genotype barley
  • A method for selecting cadium toxicity-resistant genotype barley
  • A method for selecting cadium toxicity-resistant genotype barley

Examples

Experimental program
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Embodiment 1

[0026] Choose 106 parts of different types and growth habit barley germplasm (genotypes), 150 selected seeds of each genotype, and the concentration by weight is 2% Ca(ClO) 2 After sterilizing for 20 minutes, rinse with distilled water 7 times, germinate in the dark on a sand bed in an artificial climate box at 25°C for 3 days, grow for 7 days under 15 hours of light at 20°C / 9 hours of darkness at 18°C ​​after germination, and 10 days after sowing ( Including 3 days of dark germination and 7 days of growth under 15 hours of light at 20°C / 9 hours of darkness at 18°C ​​after germination), at the 2-leaf stage, the wheat seedlings with the same growth were selected and moved to the greenhouse for pre-cultivation in cadmium-free medium for 5 days, and then cadmium-free Treatment: Set 0 (control, CK), 20μM Cd two kinds of treatment, Cd with CdCl 2 form supply. Split-plot design, cadmium treatment as the main plot, variety as the sub-plot, repeated 3 times.

[0027] The cadmium-fre...

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Abstract

The invention discloses a method for screening barley genotypes resistant to cadmium toxicity, including seed selection, sterilization, seedling cultivation, transplanting, pre-cultivation, cadmium stress treatment, and evaluation of cadmium toxicity resistance: cadmium treatment for 20 days, measuring functional leaves with a chlorophyll meter Central chlorophyll meter reading; decline rate (%)=100*(20μM Cd treatment measured value-contrast measured value) / contrast measured value; comprehensive score of each variety=chlorophyll meter reading decline rate*0.5+root number decline rate*0.1+ Root volume decrease rate*0.1+plant height decrease rate*0.1+root dry weight decrease rate*0.1+shoot dry weight decrease rate*0.1. The present invention establishes an effective index and method for barley cadmium-tolerant genotype difference screening——the reading of the chlorometer, supplemented by analysis and determination of other morphological and physiological indexes.

Description

technical field [0001] The invention belongs to the variety screening technology in the field of agriculture, in particular to a screening method for germplasm resistant to heavy metal cadmium poisoning, and is a rapid screening method for cadmium-resistant / tolerant barley breeding materials. Background technique [0002] Cadmium (Cd) is a heavy metal that is toxic to humans, animals and plants. Due to various reasons such as the discharge of industrial "three wastes", the increase in the amount of pesticides and fertilizers, and the poor disposal of solid waste, the cadmium content in farmland soil is increasing, and the cadmium pollution in the soil-plant-food system is becoming more and more serious, which makes the safety of agricultural products. being threatened. According to reports, rice cadmium pollution is the main problem of heavy metal pollution in Japan; the determination and analysis of edible cereal grains in Japan, South Korea and different regions of my cou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/04A01G1/00
Inventor 邬飞波陈飞董静汪芳张国平
Owner ZHEJIANG UNIV