Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method of detecting predisposition to high altitude pulmonary edema

A high-altitude pulmonary edema and susceptibility technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of carrying PAC infeasible, not included, septic shock, etc.

Active Publication Date: 2007-01-17
COUNCIL OF SCI & IND RES
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sometimes, inadequate inhalation can lead to hypotension or even septic shock in the patient
[0015] 2. Due to bad weather and rough mountain roads, it is often impossible for HAPE patients to go downhill immediately (AnandIS et al. 1998, Hackett PH et al. 2001)
[0016] 3. Carrying a PAC sometimes seems unfeasible due to overloading issues
[0017] 4. The reported polymorphisms associated with HAPE are not unique to this disease, but also exist in blood pressure-increasing diseases such as diabetes, coronary artery disease, hypertension, and myocardial infarction (Monti LD et al. 2003, ViaM et al. 2003)
Also, the study did not include HA natives (high altitude dwellers), that is, people who live happily in the same environment in which the disease occurs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of detecting predisposition to high altitude pulmonary edema
  • Method of detecting predisposition to high altitude pulmonary edema
  • Method of detecting predisposition to high altitude pulmonary edema

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Identification of marker genes

[0091] Considering the important function of NO in NA, iNOS (inducible nitric oxide synthase) was selected as the candidate gene in this study.

Embodiment 2

[0093] Selection of research subjects

[0094] The clinical severity of HAPE was assessed using the Lake Louise acute mountain sickness (AMS) scoring system. Briefly, the patients were evaluated for five symptoms: headache, gastrointestinal upset, fatigue, weakness, or fatigue and weakness, vertigo, dizziness, or dizziness, and difficulty sleeping. Changes in mental status, ataxia, and peripheral edema are also evaluated. Each symptom is rated on a scale of 0-3. 0 means no symptoms; 1, mild symptoms; 2, moderate symptoms; 3, severe symptoms. The HAPE score is the sum of all 8 symptoms, and the HAPE score > 6 is the patient (Anand IS et al. 1998). Lowland residents (LL) did not find any of the above symptoms even after induction at high altitude for at least three times. High Altitude (HA) Residents are permanent residents of HA from ancient times.

Embodiment 3

[0096] Extraction of leukocyte genomic DNA:

[0097] Genomic DNA of blood cells was extracted by salting out method. After lysing red blood cells in the presence of high salt, they were treated with Nuclei Lysis Buffer (NLB). A modified salting-out method was used to precipitate proteins and extract DNA from peripheral blood leukocytes. Measure the optical density of the sample at a wavelength of 260nm to determine the concentration of DNA (Miller SA et al. 1988).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a method for the detection of predisposition to high altitude pulmonary edema (HAPE). It particularly relates to allelic variants of iNOS (inducible nitric oxide synthase) gene, which has been found to be related with the prevalence of HAPE.

Description

technical field [0001] The present invention relates to methods of detecting susceptibility to high altitude pulmonary edema (HAPE). Specifically it involves the use of allelic variants of the iNOS (inducible nitric oxide synthase) gene that have been found to be associated with the prevalence of HAPE. Background of the invention [0002] High altitude pulmonary edema (HAPE) is a noncardiogenic form of pulmonary edema that occurs within 24 hours of rapid ascent to altitudes above 4000m in approximately 10% of randomly selected climbers. Similar phenomena were observed in lowland participants above 3000 m for various commercial reasons. Even higher rates are shown in susceptible HAPE subjects, approximately 60% having had the disease in the past (Houston CS et al 1960, Bartsch P et al 1997, 1990). HAPE is effectively prevented by prophylactic use of vasodilators or slow ascent. However, HAPE remains the most common cause of death due to exposure to high altitude during lon...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor A·Q·M·帕夏A·阿赫桑
Owner COUNCIL OF SCI & IND RES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com