Method of detecting predisposition to high altitude pulmonary edema

A high-altitude pulmonary edema and susceptibility technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of carrying PAC infeasible, not included, septic shock, etc.

Active Publication Date: 2007-01-17
COUNCIL OF SCI & IND RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Sometimes, inadequate inhalation can lead to hypotension or even septic shock in the patient
[0015] 2. Due to bad weather and rough mountain roads, it is often impossible for HAPE patients to go downhill immediately (AnandIS et al. 1998, Hackett PH et al. 2001)
[0016] 3. Carrying a PAC sometimes seems unfeasible due to overloading issues
[0017] 4. The reported polymorphisms a...

Method used

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  • Method of detecting predisposition to high altitude pulmonary edema
  • Method of detecting predisposition to high altitude pulmonary edema
  • Method of detecting predisposition to high altitude pulmonary edema

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Identification of marker genes

[0091] Considering the important function of NO in NA, iNOS (inducible nitric oxide synthase) was selected as the candidate gene in this study.

Embodiment 2

[0093] Selection of research subjects

[0094] The clinical severity of HAPE was assessed using the Lake Louise acute mountain sickness (AMS) scoring system. Briefly, the patients were evaluated for five symptoms: headache, gastrointestinal upset, fatigue, weakness, or fatigue and weakness, vertigo, dizziness, or dizziness, and difficulty sleeping. Changes in mental status, ataxia, and peripheral edema are also evaluated. Each symptom is rated on a scale of 0-3. 0 means no symptoms; 1, mild symptoms; 2, moderate symptoms; 3, severe symptoms. The HAPE score is the sum of all 8 symptoms, and the HAPE score > 6 is the patient (Anand IS et al. 1998). Lowland residents (LL) did not find any of the above symptoms even after induction at high altitude for at least three times. High Altitude (HA) Residents are permanent residents of HA from ancient times.

Embodiment 3

[0096] Extraction of leukocyte genomic DNA:

[0097] Genomic DNA of blood cells was extracted by salting out method. After lysing red blood cells in the presence of high salt, they were treated with Nuclei Lysis Buffer (NLB). A modified salting-out method was used to precipitate proteins and extract DNA from peripheral blood leukocytes. Measure the optical density of the sample at a wavelength of 260nm to determine the concentration of DNA (Miller SA et al. 1988).

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Abstract

The present invention relates to a method for the detection of predisposition to high altitude pulmonary edema (HAPE). It particularly relates to allelic variants of iNOS (inducible nitric oxide synthase) gene, which has been found to be related with the prevalence of HAPE.

Description

technical field [0001] The present invention relates to methods of detecting susceptibility to high altitude pulmonary edema (HAPE). Specifically it involves the use of allelic variants of the iNOS (inducible nitric oxide synthase) gene that have been found to be associated with the prevalence of HAPE. Background of the invention [0002] High altitude pulmonary edema (HAPE) is a noncardiogenic form of pulmonary edema that occurs within 24 hours of rapid ascent to altitudes above 4000m in approximately 10% of randomly selected climbers. Similar phenomena were observed in lowland participants above 3000 m for various commercial reasons. Even higher rates are shown in susceptible HAPE subjects, approximately 60% having had the disease in the past (Houston CS et al 1960, Bartsch P et al 1997, 1990). HAPE is effectively prevented by prophylactic use of vasodilators or slow ascent. However, HAPE remains the most common cause of death due to exposure to high altitude during lon...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor A·Q·M·帕夏A·阿赫桑
Owner COUNCIL OF SCI & IND RES
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