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Method of fast purifying and preparing Fab fragment antibody

A process method and fragment technology, which are applied in the fields of rapid purification and preparation of Fab fragment antibodies, protein purification, and rapid preparation of humanized/human-mouse chimeric fragment antibodies, can solve problems such as the scope of application of the method without further discussion, and achieve Small difference in results, saving time, good stability

Active Publication Date: 2007-02-07
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the test scale of Gresham T. Weatherly has reached the scale of pilot production, but the scope of application of this method (HCIC) is not further discussed in the above-mentioned literature, and there are certain limitations

Method used

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  • Method of fast purifying and preparing Fab fragment antibody
  • Method of fast purifying and preparing Fab fragment antibody
  • Method of fast purifying and preparing Fab fragment antibody

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0038] According to the technical scheme of the present invention, the applicant used a three-step method of membrane filtration-hydrophobic chromatography I-hydrophobic chromatography II in the preparation process of the anti-human liver cancer Fab fragment antibody to purify the target product.

[0039] Among them, membrane filtration is to remove cells and cell debris in the medium supernatant, and hydrophobic chromatography I is to use hydrophobic chromatography with a lower resolution to efficiently capture the Fib fragment antibody in the large-scale culture supernatant of yeast cells and concentrate the target Product; Hydrophobic chromatography II uses hydrophobic chromatography with high resolution and separation, and obtains Fab fragment antibodies with a purity of not less than 90% by controlling the binding and separation conditions of hydrophobic chromatography.

[0040] Membrane filtration removes tangible substances such as cells and cell debris in the medium sup...

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Abstract

the invention discloses a rapid preparing method of segment antibody, which comprises the following steps: removing cell and cell fragment in the medium supernatant through film filtering method; capturing high-effective segment antibody through low-distinguishing rate; condensing object product; obtaining the product with not less than 90% purity; controlling the entire course within 12 h; fitting for purifying culture manufacturing in the eukaryotic cell and prokaryote.

Description

technical field [0001] The invention belongs to the protein purification technology in the technical field of bioengineering, and mainly relates to the secreted fragment antibody produced by large-scale culture of prokaryotic cells and eukaryotic cells, especially a process method for rapid purification and preparation of Fab fragment antibody, which is suitable for therapeutic use The production and preparation of antibody drugs are especially suitable for the rapid preparation of humanized / human-mouse chimeric fragment antibodies. Background technique [0002] Antibody therapy has become an important means of biological treatment of tumors, epidemics and infectious diseases today. Antibody drugs have become a large class of new therapeutic agents in the international pharmaceutical market due to their high specificity, effectiveness and safety. With the new structural design, the in vivo pharmacokinetics are improved, the scope of immunology is broaden...

Claims

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Application Information

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IPC IPC(8): C07K1/36C07K1/34C07K1/16C07K16/46C07K16/18
Inventor 米力唐浩申小梅
Owner FOURTH MILITARY MEDICAL UNIVERSITY