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Method for synchronous fermentation of bacillus liquid for use in microbial manure

A technology of microbial fertilizer and bacillus, which is applied in the field of simultaneous fermentation of bacillus liquid, can solve the problems of uneven growth of bacteria, different growth starting points, and difficulty in production control, so as to reduce the probability of pollution and improve product quality. , Conducive to the effect of production control

Active Publication Date: 2010-09-15
BEIJING DANLU INDAL
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects that the seed liquid inoculated by the existing microbial fermentation method is easy to cause different growth starting points, the growth speed of the bacteria is uneven, several generations live together, which brings difficulties to production control, and the defect of low sporulation rate , thereby providing a method of synchronous fermentation of the bacillus liquid used for microbial fertilizers that can form spores synchronously under large-scale liquid fermentation conditions, increase the number and ratio of spore formation, and shorten the production cycle

Method used

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  • Method for synchronous fermentation of bacillus liquid for use in microbial manure

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Embodiment 1

[0023] With the nutrient solution of bacillus megaterium (by weight percentage, starch 3%, bean cake powder 1%, corn steep liquor 0.1%, K 2 HPO 4 0.5%, (NH 4 ) 2 SO 4 0.2%, CaCO 3 0.5%, pH7.2-7.5) 300 kilograms add the fermenter of the external heat exchange jacket that has agitator, be warmed up to 121 ℃, pressure reaches 0.11MPa, heat preservation and pressure-holding 30 minutes carry out sterilization. Then slowly lower the temperature to 90°C, and at the same time reduce the pressure in the tank to normal pressure. In the normal working state of the fermentation workshop, light the fire ring around the inoculation port of the tank, and open the inoculation cover of the fermentation tank under the protection of the flame. , quickly add pure bacillus megaterium liquid seeds into the fermenter, and then close the inoculation cover to make the tank body airtight. Turn on the agitation, keep the tank temperature at 90° C. and normal pressure for 15 minutes, and carry out t...

Embodiment 2

[0027] With the nutrient solution of bacillus licheniformis (by weight percentage, starch 0.5%, K 2 HPO 4 0.2%, (NH 4 ) 2 SO 4 0.1%, MgSO 4 0.05%, Yeast Extract 0.1%, CaCO 3 0.1%, pH7.0-7.3) 300 kilograms add the fermenter of the external heat exchange jacket that has agitator, be warmed up to 121 ℃, pressure reaches 0.11MPa, heat preservation and pressure-holding 35 minutes carry out sterilization. Then slowly lower the temperature to 90°C, and at the same time reduce the pressure in the tank to normal pressure. In the normal working state of the fermentation workshop, light the fire ring around the inoculation port of the tank, and open the inoculation cover of the fermentation tank under the protection of the flame. , quickly add pure Bacillus licheniformis liquid seeds into the fermenter, and then close the inoculation cover, so that the tank body is in an airtight state. Turn on the agitation, keep the tank temperature at 85° C. and normal pressure for 20 minutes,...

Embodiment 3

[0031] The culture medium of Bacillus lateralis (by weight percentage, peptone 0.5%, bean cake powder 0.2%, yeast powder 0.2%, starch 1%, sucrose 0.5%, K 2 HPO 4 0.2%, MgSO 4 0.05%, CaCO 30.01%, pH7.0-7.5) 300 kilograms add the fermenter of the external heat exchange jacket that has agitator, be warmed up to 121 ℃, pressure reaches 0.11MPa, heat preservation and pressure-holding 35 minutes carry out sterilization. Then slowly lower the temperature to 88°C, and at the same time reduce the pressure in the tank to normal pressure. In the normal working state of the fermentation workshop, light the fire ring around the inoculation port of the tank, and open the inoculation cover of the fermentation tank under the protection of the flame. , quickly add the pure Bacillus lateralis sporogenes liquid seeds into the fermenter, and then close the inoculation cover, so that the tank body is airtight. Turn on the agitation, keep the tank temperature at 88° C. and normal pressure for 1...

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Abstract

The invention discloses a synchronous ferment method of bacillus liquid through microbe fertilizer, which is characterized by the following: seeding spore seed liquid on the seed tank directly; disposing under high temperature; shortening ferment period by 6h; controlling the quantity and proportion of spore easily.

Description

technical field [0001] The invention relates to a method for synchronous fermentation of bacillus liquid used for microbial fertilizers. Background technique [0002] Fertilizer is an important means of agricultural production. The application of microbial fertilizers can not only ensure the output of agricultural products, but also significantly improve the quality of products, reduce environmental pollution, improve soil fertility, improve soil, and prevent and control soil diseases and insect pests. New fertilizer for green food. At present, it has been vigorously promoted in our country and has shown a bright application prospect. For this reason, the Ministry of Agriculture of the People's Republic of China has formulated and issued "Microbial Fertilizer Industry Standard NY-227-94", and other microbial fertilizer series standards, and clearly stipulated the types of effective microorganisms as fertilizers and their quality standards. Among them, the number of viable ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/07
Inventor 高昭远林淑华
Owner BEIJING DANLU INDAL
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