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Rice blast gene related with virulence and its use

A rice blast fungus and gene technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve problems such as affecting growth and yield, and seedling death.

Inactive Publication Date: 2010-05-05
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If rice blast occurs at the seedling stage, it will seriously affect the growth and yield, and what is more, it will cause the death of the seedlings

Method used

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  • Rice blast gene related with virulence and its use
  • Rice blast gene related with virulence and its use
  • Rice blast gene related with virulence and its use

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Experimental program
Comparison scheme
Effect test

Embodiment

[0035] 1. Obtaining of T-DNA insertion mutants of MgURO-D gene:

[0036] This mutant was obtained by the Agrobacterium tumefaciens-mediated transformation method (ATMT) (Caroline et al., 2005).

[0037] 1.1 Cultivation of Agrobacterium

[0038] Pick a single colony of Agrobacterium tumefaciens strain Agl-1 (Mullins, 2001) containing the plasmid pBHt2 (Mullins, 2001), minimal medium (MM; Hooykaas et al., 1979), 50ug / ml kanamycin, 10ug / ml rifampicin, 250rpm, shake culture at 28°C for 48h; centrifuge at 4000rpm for 5 minutes, discard the supernatant, resuspend in induction medium (IM; Bundock et al., 1995), centrifuge at 4000rpm for 5 minutes, discard the supernatant; IM (containing 300 uM acetosyringone) medium resuspended, 28 ° C, 250 rpm shaking shake bacteria 6h.

[0039] 1.2 Acquisition of Magnaporthe grisea spores

[0040] In this study, the pathogenic strain-Y34 (provided by China Agricultural University) isolated from the field of Yunnan, China was selected as the res...

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Abstract

MgURO-D relating to yeast, human ecological uroporphyrin protodecarboxylating enzyme URO-D gene, its expression carrier, host and usage are disclosed. The gene code contains 367 amino acid, 4 intronsand 5 exons. It can be used to prevent rice blast and as target of medicine to prevent plant pest.

Description

technical field [0001] The invention relates to the field of plant disease prevention and control, more specifically, the invention relates to a gene related to the pathogenicity of Magnaporthe grisea and having higher homology with yeast, human and other biological uroporphyrinogen decarboxylase genes , the MgURO-D gene. The present invention also relates to the application of the gene in plant disease control and as the target of medicine for plant disease control. Background technique [0002] Rice blast, caused by Magnaporthe grisea, is the most serious disease of cultivated rice, causing 10% to 30% loss of rice yield every year. In addition to rice, this filamentous fungus can also infect many other species of Poaceae, such as wheat, scorpion and other crops (Ekwamu, 1991; Igarashi et al., 1986). It invades into plant cells through a specialized infection structure, the appressorium. The appressorium is a dome-shaped, melaninized cell wall that develops from germinat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/31C12N15/52C12N9/00C07K14/37C12N15/63C12N15/82A01N63/04
Inventor 何朝族李桂华
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI