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The euphorbia lunulate bge extractant and production method and use thereof

A technology of cat's eye grass and its use, applied in the field of cat's eye grass extract and its preparation and use, to achieve the effect of overcoming toxic and side effects

Inactive Publication Date: 2011-07-06
NIHON UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of various compounds remains to be further clarified

Method used

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  • The euphorbia lunulate bge extractant and production method and use thereof
  • The euphorbia lunulate bge extractant and production method and use thereof
  • The euphorbia lunulate bge extractant and production method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) The crude extraction method and product of cat's eye grass:

[0030] Take 9.0 kg of cat's eye grass (Euphorbia lunulata Bge), extract three times with 70% acetone (27 L), concentrate the extract, add water to the obtained extract to make it a suspension, and extract with chloroform, ethyl acetate, and n-butanol successively. The NO formation inhibition test and MTT test were carried out on these three extracts respectively, and the sample concentration was 100 μg / ml. The results are shown in Table 1:

[0031] Table 1. Yield, NO production inhibition rate and MTT toxicity of each extract

[0032] Extracts

Yield (g)

NO production inhibition rate

MTT toxicity

Chloroform extract

65.25

89.8％

have

Ethyl acetate extract

52.43

82.1％

none

n-butanol extract

86.51

33.1％

none

[0033] Select the ethyl acetate extract with high inhibition rate of NO formation and no MTT toxicity to c...

Embodiment 2

[0045] The physical and chemical parameters of the three compounds obtained by separation and purification were analyzed, and the main data are shown in Tables 3, 4 and 5.

[0046] Table 3. Basic physical and chemical properties of compounds 1-3:

[0047]

[0048] Table 4. Compounds 1 and 2 1 H and 13 C-NMR data (500MHz, dissolved in DMSO-d 6 )

[0049]

[0050] Table 5. 1H and 13C-NMR data of compound 3 (300MHz, dissolved in acetone)

[0051]

[0052]

[0053] The structures of compounds 1-3 are as follows:

[0054]

[0055] Example 3 .NO production inhibitory activity of cat's eye extract

Embodiment 3

[0056] Materials used:

[0057] RAW264.7 cells (macrophages, transformed with Abelson leukemia virus) were obtained from ATCC (American Type Culture Collection), USA. Ham's F12 medium containing 10% FBS in 5% CO 2 , cultured at 37°C.

[0058] IFN-γ: Genzyme / Techne Company; LPS: Sigma Company; Sulphonamide: Wako Company; N-1-Naphthethylene-diamine Dihyrochloride: Wako Company Griess Reagent: (1) N- 1-Naphthylethylenediamine hydrochloride was dissolved in 5ml of water for injection; (2) 250 μl of phosphoric acid was added to 50 mg of sulfonamide in 5 ml of water for injection.

[0059] Instrument: Microtiter plate reader (Microtiter Plate Reader Model 3550, BIO-RAD).

[0060] Specific methods and results:

[0061] Take 50ml of RAW264.7 cells and put them in a Falcon tube for centrifugation (1000rpm, 3min, 4°C) to pellet the cells, remove the supernatant with a pipette, add 10ml of new medium, and make it suspended. (adjust density to 1.5×10 5 per ml) into 96-well plate, 20...

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Abstract

The present invention relates to the method of extracting active compounds from medicinal plant Euphorbia Lunulate Bge, consists of the following steps: The Acetone extractants of Euphorbia Lunulate Bge are abstracted and crude extracted by using organic solvents; the organic solvents extractants are subjected to inhibit production of NO activity test, and further isolate the extractants which have high production of NO inhibiting activity and no toxicity; then the isolated compositions are subjected to inhibit production of NO activity test again, and further refine the compositions which have high production of NO inhibiting activity; the achieved single compound is subjected to inhibit production of NO activity test and physical and chemical identification so as to definition its structure. The achieved compound has the activity of inhibiting production of NO, in which the structures of compound 1-3 are following formulas.

Description

technical field [0001] The invention relates to the separation and extraction of anti-inflammatory active substances from the medicinal plant Euphorbia Lluaulata Bge and its preparation method and application. Background technique [0002] Daily inflammatory diseases that plague people include joint inflammation such as osteoarthritis, rheumatoid arthritis, rheumatoid spondylitis, gouty arthritis, etc.; inflammatory skin diseases such as eczema, psoriasis, dermatitis, etc.; Uveitis, conjunctivitis, etc.; lung diseases such as asthma, bronchitis, acute respiratory distress syndrome, etc.; bacteremia, that toxemia, aphthous ulcers, gingivitis, pancreatitis, etc.; Ileitis, atrophic gastritis, ulcerative colitis, celiac disease, peptic ulcer, irritable bowel syndrome such as mucosal inflammation caused by Helicobacter pylori infection or gastrointestinal tract caused by non-steroidal anti-inflammatory drugs illness and so on. [0003] Regarding the mechanism of various inflamm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/35A61K31/70A61K36/47A61P1/02A61P1/04A61P29/00A61P37/00
CPCA61K31/35A61K31/70
Inventor 王立岩北中进
Owner NIHON UNIVERSITY
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