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Bacterial antigens and vaccine compositions

a technology of antigens and compositions, applied in the field of antigenic h . pylori polypeptide, can solve the problems of inadequate or directed spontaneous immune response or other problems

Inactive Publication Date: 2002-11-14
BOLIN INGRID +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason for this is probably that the spontaneously induced immune-response is inadequate or directed towards the wrong epitopes of the antigens.

Method used

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  • Bacterial antigens and vaccine compositions
  • Bacterial antigens and vaccine compositions
  • Bacterial antigens and vaccine compositions

Examples

Experimental program
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Effect test

example 1

Cloning and Expression of a 29 kDa Polypeptide From H. pylori

1.1. Bacterial Strains, Vectors and Growth Conditions

[0053] H. pylori CCUG 17874 (=NTCC 11637) was grown on horse blood agar plates in an rnicroaerophilic atmosphere. E. coli strains XL1-Blue MRF' and XLOLR (Stratagene, La Jolla, Calif.) were used as host strains for cloning experiments and were grown in Luria-Bertani broth (LB) or NZY medium supplemented with 0.2% maltose and 10 mM MgSO.sub.4 when used for lambda infection. The lambda expression vector ZAP Express.TM. and its phagemid derivative pBK-CMV were obtained from Stratagene.

1.2. DNA Techniques

[0054] Chromosomal DNA from H. pylori 17874 was prepared by suspending bacteria from plates incubated for 48 h in 50 mM Tris-Cl, pH 8.0, 25% sucrose, 50 mM EDTA containing 10 mg / ml lysozyme, and 5 ng / ml DNase-free RNase (Boehringer Mannheim Scandinavia AB, Bromma, Sweden). The suspension was incubated for 10 min at +37.degree. C. An equal volume of lysis buffer (0.4% Triton ...

example 2

Kinetics of Expression of the 29 kDa Polypeptide during Various Culture Conditions

[0071] Two strains of H. pylori were used, namely CCUG 17874 (a laboratory strain) and Hel 73 (recently isolated from a patient suffering from duodenal ulcer). Cultivation was performed on blood agar plates, as well as in Brucella Broth supplemented with cyclodextrin. All cultures were incubated in a microaerophilic atmosphere consisting of 5% O.sub.2, 10% CO.sub.2 and 85% N.sub.2. Bacteria were harvested after 2, 4 and 7 days, washed once in PBS and kept at -20.degree. C. for subsequent analysis. The expression of the 29 kDa surface polypeptide was analysed by inhibition-ELISA employing specific monoclonal antibodies as previously used for detection of E. coli surface antigens (Lopez-Vidal, Y and Svennerholm, A- M., J. Clin. Microbiol. 28, 1906-) against the polypeptide. These antibodies were also used in immunoelectron microscopy.

[0072] When CCUG 17874 had been cultivated for 7 days, on blood agar pl...

example 3

Antibody Responses Against the 29 kDa Polypeptide

[0073] Antibody responses against the 29 kDa polypeptide were determined in sera and gastric aspirates from patients with duodenal ulcers (n=19), in asymptotic H. pylori carriers (n=18) and in non-infected age-matched controls (n=20).

[0074] Antibody levels against the 29 kDa polypeptide were tested in gastric aspirates and in sera from the three groups of subjects, by means of different ELISA methods. A majority of the infected subjects had significantly higher levels, compared with the healthy control persons, of specific antibodies against the 29 kDa polypeptide both in serum and in gastric aspirates. Antibody titers in asymptotic carriers were comparable to those of the symptomatic patients.

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Abstract

The present invention relates to recombinant polypeptides which constitute Helicobacter pylori surface-exposed antigens with an approximate molecular weight of 29 kDa. The invention furthermore provides nucleic acid molecules coding for the said polypeptides, as well as vectors and host cells comprising such nucleic acid molecules. The said recombinant polypeptides are useful for the diagnosis of H. pylori infections and for the manufacture of vaccine compositions which will elicit a protective immune response against such infections, said vaccine compositions being suitable for both therapeutic and prophylactic use.

Description

[0001] The present invention provides recombinant polypeptides which constitute Helicobacter pylori antigens, said antigens being expressed on the surface of both dividing (bacillary) forms as well as resting (coccoid) forms of H. pylori, and giving rise to both systemic and local (mucosal) production of antibodies. The invention furthermore provides nucleic acid molecules coding for the said polypeptides, as well as vectors and host cells comprising such nucleic acid molecules. The said recombinant polypeptides are useful for the diagnosis of H. pylori infections and for the manufacture of vaccine compositions which will elicit a protective immune response against such infections, said vaccine compositions being suitable for both therapeutic and prophylactic use.[0002] The gram-negative bacterium Helicobacter pylori is an important human pathogen, involved in several gastroduodenal diseases. Colonization of gastric epithelium by the bacterium leads to active inflammation and progre...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/00C12N15/09A61K39/106A61P31/12C07K14/205C12N1/21C12N15/11C12P21/02C12R1/01C12R1/19G01N33/569
CPCC07K14/205A61K39/00A61P31/12
Inventor BOLIN, INGRIDSVENNERHOLM, ANN-MARI
Owner BOLIN INGRID
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