Synthetic peptides that bind to the hepatitis B virus core and E antigens
a technology of hepatitis b virus and e antigen, applied in the field of synthetic peptides that bind to the hepatitis b virus core and e antigen, can solve the problems of varying the degree of acute liver injury, and achieve the effect of modulating the host immune respons
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[0039] The mAbs that were used to create the binding partners specific for HBcAg and / or HBeAg were made by conventional techniques, as described above, using recombinant peptides. Full length recombinant HBcAg (rHBcAg) encompassing residues 1-183 was produced in Escherichia coli, as previously described. (Schodel et al., J Biol Chem, 268:1332-7 (1993), herein expressly incorporated by reference in its entirety). A truncated recombinant form of HBeAg containing nine residues of pre-core and the 150 first residues of HBcAg was also made. Further, a non-structural 3 protein (NS3) of the hepatitis C virus (HCV) was also made to serve as a control. (Jin and Peterson, Arch. Biochem. Biophys., 323:47-53 (1995), herein expressly incorporated by reference in its entirety). Another control peptide, an analogue of HBcAg (ΔHBcAg), wherein region 76-85 was replaced by an irrelevant sequence, was also made.
[0040] Balb / c and CBA mice (purchased from BK Universal, Sollentuna, Sweden) were immunize...
example 2
[0042] To determine the reactivity and specificity of a mAb, recombinant proteins or fragments thereof (e.g., HBcAg, HBeAg, ΔHBcAg, denatured HBcAg, or NS3 proteins) were passively adsorbed at 10 μg / ml to 96 well microtiter plates in 50 mM sodium carbonate buffer, pH 9.6, overnight at 4° C. Serial dilutions of mAbs were made in phosphate buffered saline (PBS) containing 2% goat serum (Sigma Chemicals, St Louis, Mo.), and 0.05% Tween 20 (PBS-GT). The various dilutions were then incubated on the plates for 60 minutes. Bound mAbs were detected either by rabbit anti-mouse IgG (Sigma), or rabbit anti-mouse IgG1, IgG2a, IgG2b or IgG3 (Sigma) followed by a peroxidase labeled goat anti-rabbit IgG (Sigma). The plates were developed by incubation with dinitro-phenylene-diamine (Sigma) and the absorbance at 490 nm was determined. Additionally, the reactivity in Abbott anti-HBc and HBe IMX assays was determined according to the manufacturer's instructions. The results of these studies are provi...
example 3
[0044] To determine the protein sequence of an antibody binding domain, total cellular mRNA was extracted using magnetic beads coated with oligo-dT25 (Dynal A. S, Oslo, Norway). The variable domains of the heavy (VH) and light (VL) chains of mAbs were amplified from cDNA by the Polymerase Chain Reaction (PCR) using the recombinant phage antibody system (Pharmacia Biotech, Uppsala, Sweden). The amplified cDNA fragments were directly ligated to the TA cloning vector pCR 2.1 (Invitrogen, San Diego, USA) as described. (Zhang et al., Clin. Diagn. Lab. Immunol., 7:58-63 (2000), herein expressly incorporated by reference in its entirety). The DNA sequences were determined by an automated sequencer (ALF express, Pharmacia, Uppsala, Sweden) as described. (Zhang et al., Clin. Diagn. Lab. Immunol., 7:58-63 (2000), herein expressly incorporated by reference in its entirety). From the cDNA sequence, a corresponding protein sequence was deduced. The protein sequences deduced from VH cDNA clones o...
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