Macromolecules comprising a thioether cross-link
a technology of macromolecules and thioethers, which is applied in the field of compositions comprising macromolecules, can solve the problems of the polyclonal nature of natural immunoglobulins, which hinders use, especially in therapy, and achieves the effects of increasing increasing the amount of said antibody species, and decreasing the amount of an antibody
Inactive Publication Date: 2009-01-29
MEDIMMUNE LLC
View PDF0 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
The present invention provides macromolecules, such as polypeptides and antibodies, that have a thioether cross-link. The thioether cross-link is a single thioether bond between two residues of the macromolecule. The macromolecule can be a polypeptide, an antibody, or a fragment thereof. The thioether cross-link can link any two residues of the macromolecule. The thioether cross-link can be at any position in the macromolecule. The invention also provides a composition comprising the macromolecule or antibody with the thioether cross-link. The thioether cross-link can specifically bind to antigens of respiratory syncytial virus (RSV) or human metapneumovirus (hMPV). The invention also provides a method for making the macromolecule or antibody with the thioether cross-link.
Problems solved by technology
However, such uses, especially in therapy, have been hindered by the polyclonal nature of natural immunoglobulins.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0051]The present invention is based, in part, on the discovery and characterization of a non-reducible thioether bridge between the heavy and light chains of different monoclonal antibodies. Accordingly, the invention provides macromolecules comprising a thioether cross-link, wherein the thioether cross-link is a thioether comprising a single thioether bond linking two residues of a macromolecule.
5.1. DEFINITIONS
[0052]As used herein, the term “isolated” in the context of a proteinaceous agent (e.g., peptide, polypeptide, fusion protein or antibody) refers to a proteinaceous agent which is substantially free of cellular material or contaminating proteins from the cell or tissue source from which it is derived, or substantially free of chemical precursors or other chemicals when chemically synthesized. The language “substantially free of cellular material” includes preparations of a proteinaceous agent in which the proteinaceous agent is separated from cellular components of the cell...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The present invention provides macromolecules comprising at least one thioether cross-link. A thioether cross-link comprising a single thioether bond between two residues of a macromolecule. The macromolecules of the invention can display enhanced stability, pharmaceutical properties and functional properties. In particular, the invention provides an isolated antibodies comprising at least one thioether cross-link that specifically bind to particular antigens. The present invention also provides a composition comprising a macromolecule substantially free of a denaturing reagent, wherein the macromolecule comprises at least one thioether cross-link. In addition, the present invention provides a method for producing the macromolecules and compositions of the invention.
Description
[0001]This application claims the benefit of priority of U.S. provisional application Nos. 60 / 661,724 and 60 / 699,138, filed Mar. 14, 2005, and Jul. 13, 2005, respectively, the contents of which are hereby incorporated by reference in their entireties.1. FIELD OF THE INVENTION[0002]The present invention provides compositions comprising macromolecules, for instance, polypeptides, e.g., an antibody, that comprise at least one thioether cross-link. This novel class of macromolecules can display enhanced stability, pharmaceutical properties and functional properties. The invention also provides isolated antibodies comprising at least one thioether cross-link that specifically bind to particular antigens. In addition, the present invention provides methods for producing the macromolecules and compositions of the invention.2. BACKGROUND OF THE INVENTION[0003]Antibodies play a vital role in our immune response. They can inactivate viruses and bacterial toxins, and are essential in recruitin...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(United States)
IPC IPC(8): C07K16/00C07K16/08C07K16/18C07K16/24
CPCC07K16/00C07K16/1027C07K2317/52C07K2317/624C07K2317/24
Inventor TOUS, GUILLERMO I.SCHENERMAN, MARKWEI, ZIPING
Owner MEDIMMUNE LLC