Polyclonal-monoclonal elisa assay for detecting n-terminus pro-bnp

a polyclonal and monoclonal technology, applied in the field of ntprobnp protein elisa assay, can solve the problems of antibody sensitivity generally too low to reach the necessary sensitivity in a test procedure, antibody which generally cannot bind to the whole molecule, and the sandwich test described in wo 93/24531 in a sample cannot be performed as described, so as to achieve accurate prediction of mortality
US20090325195A1Inactive Publication Date: 2009-12-31NEXUS DX
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
NEXUS DX
Publication Date
2009-12-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure circulating NT-proBNP in human plasma. In order to obtain antibodies with specific binding properties for targeted amino acid sequences within human proBNP, recombinant human proBNP (or rhproBNP) was expressed and purified for use as an immunogen. Polyclonal antibodies (PAb) to specific amino acid sequences were subsequently purified from goat serum by sequential affinity purification. Monoclonal antibodies were raised against specific polypeptides. Recombinant human NT-proBNP (or rhNT-proBNP) was expressed and purified in order to obtain material for use in calibration of a quantitative method for measurement of human NT-proBNP.
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Description

CROSS REFERENCE TO RELATED APPLICATIONS

[0001] This application is a continuation of U.S. patent application Ser. No. 10 / 359,051, filed on Feb. 4, 2003, which is a continuation of U.S. patent application Ser. No. 10 / 300,733, filed on Nov. 18, 2002, the contents of which are herein incorporated by reference.FIELD OF THE INVENTION

[0002] This invention relates to an NT-proBNP protein ELISA assay procedure and test kit which is a specific and sensitive in vitro assay for measuring the concentration of NT-proBNP in bodily fluids, particularly human plasma. The invention particularly relates to an NT-proBNP protein ELISA assay having a particularly high diagnostic specificity, whereby the assay is particularly designed to be predictive of mortality as a result of congestive heart failure.BACKGROUND OF THE INVENTION

[0003] B-type natriuretic peptide (Brain natriuretic peptide, BNP) belongs to the family of structurally similar, but genetically distinct natriuretic peptides (NPs) first described...

Claims

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