Agent for increasing testosterone level
a testosterone and enhancer technology, applied in the field of testosterone enhancers, can solve the problems of rapid increase in the blood testosterone level, poor memory, and deterioration of strength, micturition, etc., and achieve the effect of less stress on the patien
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example 1
[0044]Blood testosterone enhancement effect of the testosterone enhancer of the present invention was studied using ordinary rats. An ordinary rat is a rat in which extreme vitamin K deficiency does not occur even when feed does not include vitamin K because the rat absorbs vitamin K produced by bacteria in its intestinal tract. The ordinary rat is a model extrapolating to a human living an ordinary life.
Materials and Methods
I Preparation of Model Animals
Breeding Experimental Animals
[0045]Experimental animals and breeding conditions are as follows:
Experimental animals: ordinary rats (Wistar / Std, eight-week-old males)
Breeding environment: bred in a breeding room set to a temperature of 23° C., humidity of 50±5%, and a 12-hour light / dark cycle in which lights were turned on at 8 AM and turned off at 8 PM.
[0046]As experimental dietary groups, the following three groups were provided.
[0047](1) Low K dietary group (no vitamin K was added)
[0048](2) Control dietary group (vitamin K1 of 0.7...
example 2
Materials and Methods
[0107]As in Example 1, the experimental animals and breeding conditions were ordinary rats (Wistar / Std, eight-week-old males) bred in a breeding room set to a temperature of 23° C., humidity of 50±5%, and a 12-hour light / dark cycle in which lights were turned on at 8 AM and turned off at 8 PM.
[0108]As experimental dietary groups, the following three groups were provided.
(1) Control dietary group (vitamin K1 of 0.75 mg / kg was added)
(2) Vitamin K1 added dietary group (vitamin K1 of 75 mg / kg was added)
(3) MK-4 added dietary group (menaquinone-4 of 75 mg / kg was added)
[0109]Vitamin K1 was purchased from Wako Pure Chemical Industries, Ltd. Menaquinone-4 from Nisshin Pharma Inc. was used. Vitamin K1 or menaquinone-4 was added to each experimental diet to achieve the composition proportions shown in Table 1 and evenly mixed.
[0110]The breeding period was 35 days. Blood was drawn from tail veins every week at 18 o'clock. Measurements of weight, diet intake, testis vitamin...
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