Biological sample vitrification carrier and usage thereof

Abstract

The present invention provides a biological sample vitrification carrier and a method for freezing a biological sample rapidly, simply, conveniently, closely and sterilely. Specifically, the carrier comprises a main body and a sealing cap. A near end of the main body and an inner bottom surface of the sealing cap are mutually sealed to form a sealed frozen sample placing region. The frozen sample placing region is provided with a frozen sample placing platform, the main body is provided with a frozen liquid circulating channel, and the sealing cap is provided with a guide hole corresponding to the frozen liquid circulating channel, so that, when the biological sample is frozen, frozen liquid circulating in the channel contacts with an outer surface of the frozen sample placing region and cools the biological sample in the frozen sample placing region. The biological sample vitrification carrier and the method in the present invention have the advantages of safety, nontoxicity and high vitrification and rewarming operation efficiency and have wide application prospects in the field of biological sample freezing.

Description

TECHNICAL FIELD[0001]The present invention relates to the field of biological sample freezing device, specifically including providing a safe, effective and easily operated sealed freezing carrier for embryo(s) or egg cell(s) to be used in sterilized vitrification in the assisted reproductive technology field.BACKGROUND ART[0002]For artificial assisted reproductive technology, a common method for improving the success rate of in vitro fertilization and embryo transplantation is cryopreserving more embryos obtained by vitro fertilization or eggs obtained from female in the −196° C. liquid nitrogen for short-term or long-term selective embryo transplantation. The clinical significancy of vitrification of biological sample is that the cryopreserved embryos or cells will stop development and be preserved for decades in coolant by a long-term preservation in a coolant such as liquid nitrogen, besides, upon rewarming the cryopreserved biological samples, one or more biologically live cell...

AI Technical Summary

Benefits of technology

This patent describes a carrier that can safely and non-toxically preserve biological samples. The carrier has a fast freezing rate which prevents damage to the biological samples during freezing. It is easy to operate and the samples can be easily recovered without losing them. The technical effects of the carrier are high efficiency, safety, and convenience.

Problems solved by technology

When the materials are subjected to cryopreservation, the contact of material at room temperature with coolant at extremely low temperature (eg. liquid nitrogen) results in Leidenfrost effect, which causes the formation of vapor blanket to block conducting of heat, thereby decreasing the cooling rate of the desired freezing material.

However, cryoprotectant of high concentration is toxic to biological samples and will result in cells with low quality after recovery.

However, the biological sample was preserved in a non-sterilized condition, resulting the risk of cross contamination and the unpredictable direct toxic effect of liquid nitrogen on cells due to the direct contact of biological sample with liquid nitrogen (Covo A, Domingo J, Perez S, et al.

However, although a sealed system for the isolation of coolant is obtained, the operation process of this type of cryopreservation carrier is still complicated since several steps such as thermoplastic sealing, etc., are needed.

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