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Methods for selecting competent oocytes and competent embryos with high potential for pregnancy outcome

a technology of competent oocytes and embryos, applied in the field of selecting competent oocytes or competent embryos, can solve the problems of difficult implementation of biomarkers directly directed to oocytes or embryos, limited prediction power of this approach, etc., and achieve the effect of high implantation ra

Inactive Publication Date: 2016-07-14
INST NAT DE LA SANTE & DE LA RECHERCHE MEDICALE (INSERM) +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent text describes a method that helps embryologists identify embryos with a poor potential for pregnancy success. This helps to avoid transferring these embryos into a patient's uterus, which can save time and resources.

Problems solved by technology

The selection of embryos with higher implantation potential has been one of the major challenges in ART.
However, the predictive power of this approach is still limited.
Legal and ethical considerations make biomarkers directly directed to oocytes or embryos difficult to implement.

Method used

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  • Methods for selecting competent oocytes and competent embryos with high potential for pregnancy outcome
  • Methods for selecting competent oocytes and competent embryos with high potential for pregnancy outcome

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example

[0095]Samples:

[0096]Cumulus cells and mature MII oocytes were collected from patients consulting for conventional IVF (cIVF) or for ICSI (male infertility). Cumulus cells were removed from a mature oocyte (MII). CCs were partially separated mechanically from the corresponding oocyte as previously described (Assou et al., 2008). Unfertilized MII oocytes were collected 21 or 44 h after insemination or after microinjection by ICSI. Cumulus cells and oocytes were frozen at −80° C. in RLT buffer (RNeasy Kit, Qiagen, Valencia, Calif.) before miRNA extraction.

[0097]Methods for Determining the Expression Level of the microRNA:

[0098]Determination of the expression level of the microRNA can be performed by a variety of techniques known in the art.

[0099]Preferably, small RNA from cumulus cells and mature MII oocyte samples was extracted after storage of samples at −80° C. in RLT RNA extraction buffer supplemented with 1 μM of 2-β-mercaptoethanol (M-3148, Sigma) as described in the manufacturer...

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Abstract

The present invention relates to a method for selecting a competent oocyte or a competent embryo.

Description

FIELD OF THE INVENTION[0001]The present invention relates to a method for selecting a competent oocyte or a competent embryo.BACKGROUND OF THE INVENTION[0002]In assisted reproductive technology (ART), pregnancy and birth rates following in vitro fertilization (IVF) attempts remain low. Indeed, 2 out of 3 IVF cycles fail to result in pregnancy (SART 2004) and more than 8 out of 10 transferred embryos fail to implant (Kovalevsky and Patrizio, 2005). In addition, more than 50% of IVF-born babies are from multiple gestations (Reddy et al., 2007). Preterm deliveries that result from multiple pregnancies caused by ART are estimated to account for approximately $890 million of U.S. health care costs annually (Bromer and Seli, 2008).[0003]The selection of embryos with higher implantation potential has been one of the major challenges in ART. This selection is currently based on morphological criteria such as growth rate, early cleavage on day-1, degree of fragmentation and blastocyst format...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/68
CPCC12Q1/6881C12Q2600/158C12Q2600/178C12N15/111C12N15/113C12N2310/141C12N2320/10
Inventor HAMAMAH, SAMIRASSOU, SAID
Owner INST NAT DE LA SANTE & DE LA RECHERCHE MEDICALE (INSERM)
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