High performance liquid chromatography method for analysis of mn diagnostic and therapeutic ligand and precursor

Abstract

A high performance liquid chromatography method for analysis of a MN diagnostic and therapeutic ligand and precursors is revealed. Polarity of eluents used during elution is changed to remove impurities. First use a first eluent with a lower ratio of acetonitrile as a mobile phase to elute analytes. Then a second eluent in which a ratio of acetonitrile is increased into 97˜99% is used to elute the analytes for at least 20 minutes. Next use the first eluent to elute the analytes for at least 60 minutes. Thus no residual impurities are left in the column and the analytes remain in the column stably. Therefore a more accurate and reproductive result is obtained.

Description

BACKGROUND OF THE INVENTIONField of the invention[0001]The present invention relates to an analytical method, especially to a high performance liquid chromatography method for analysis of a MN diagnostic and therapeutic ligand, and MN diagnostic and therapeutic ligand precursors.Descriptions of Related Art[0002]Among cancer patients, liver cancer is more common than other cancers. The factors that induce liver cancer include chronic hepatitis B, chronic hepatitis C, alcoholic hepatitis, non-alcoholic fatty liver disease, etc. The liver cancer patients are distributed over various areas including Asia, Africa, and American. Thus the liver cancer has become a major public health problem worldwide.[0003]The latest liver cancer treatment options include surgery, chemotherapy, radiotherapy, transcatheter arterial embolization, etc. Most patients at early stage without liver cirrhosis are treated by surgery while patients having liver cirrhosis are treated by orthotopic liver transplantat...

AI Technical Summary

Benefits of technology

[0007]Therefore it is a primary object of the present invention to provide a high performance liquid chromatography method for analysis of a MN diagnostic and therapeutic ligand and precursors in which a solution containing a high ratio of acetonitrile is used to wash low-polarity impurities from the column. Thus accuracy of the analysis is ensured and the possibility of the impurities residual in the column is reduced.

[0008]It is another object of the present invention to provide a high performance liquid chromatography method for analysis of a MN diagnostic and therapeutic ligand and precursors in which ultraviolet light with a wavelength of 210 nm is used to detect most of molecules eluted and the accuracy of area percentage of the detection signal is improved.

[0009]It is another object of the present invention to provide a high performance liquid chromatography method for analysis of a MN diagnostic and therapeutic ligand and precursors in which a concept of gradient elution is revealed. That means impurities in the column are washed out by eluents with changes in polarity. Thus the reliability of the analysis result is increased.

Problems solved by technology

Thus the liver cancer has become a major public health problem worldwide.

The rest patients unable to have surgery owing to liver cirrhosis and not qualified for transplantation need effective alternative treatments.

The results of the few analysis methods available now are lack of reproducibility because low-polarity impurities are unable to be washed out during analysis.

Yet impurities with low polarity are unable to be detected because the impurities are unable to be eluted from the chromatography column by the mobile phase with relatively low polarity.

On the other hand, the impurities with low polarity residual in the chromatography column may obstruct the flow through the column or affect the following HPLC analysis.

Thus the purity is underestimated.

However, the mobile phase / eluent used in this prior art is not compatible with the protective agents used during preparation of MN diagnostic and therapeutic ligand.

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