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Device for providing a hybridization chamber, and process unit and system for hybridizing nucleic acid samples, proteins, and tissue sections

Inactive Publication Date: 2005-09-20
TECAN TRADING AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0006]The object of the present invention is therefore to suggest a device, using which the quantity of specimen liquid to

Problems solved by technology

However, it takes too long until an adequately good distribution of the specimen liquid in relation to the samples immobilize

Method used

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  • Device for providing a hybridization chamber, and process unit and system for hybridizing nucleic acid samples, proteins, and tissue sections
  • Device for providing a hybridization chamber, and process unit and system for hybridizing nucleic acid samples, proteins, and tissue sections
  • Device for providing a hybridization chamber, and process unit and system for hybridizing nucleic acid samples, proteins, and tissue sections

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first embodiment

[0023]FIG. 1 shows a perpendicular longitudinal section through a device 1 according to the present invention according to a This device is used for providing a hybridization chamber 2 for hybridizing nucleic acid samples, proteins, or tissue sections on a slide 3. Device 1 is implemented so that it is movable in relation to this slide 3 (in this case, pivotable around an axis, cf. FIG. 7A), so that hybridization chamber 2 may be opened and closed by the simplest possible movement. An annular sealing surface 4 is used for sealing hybridization chamber 2 by being applied to a surface 5 of this slide 3. This sealing surface 4 may be an offset surface of device 1 which lies flat on surface 5 of slide 3 (not shown). However, an annular seal is preferably used as sealing surface 4 (shown here as an O-ring and referred to the following as seal 4), or a lip seal may, for example, also be used as an alternative to this. The device includes lines 6 for supplying and / or removing media to and...

second embodiment

[0026]FIG. 3 shows a perpendicular longitudinal section through a device 1 according to the present invention according to a Agitation device 8 is implemented here as a membrane pump and includes flow-limiting means 13, which allow a preferred flow direction (filled arrows) of the liquids moved. Such flow-limiting means 13 may include return valves having balls (shown in FIG. 3), membranes, etc. and are known per se. A second and third agitation chamber 11′,11″, which are provided with membranes 9′,9″, are preferably connected via a second and / or third agitation line 12′,12″ to hybridization chamber 2. If a pressure surge now pushes first membrane 9 into first agitation chamber 11, this pulse (thanks to flow-limiting means 13) is only transmitted to the specimen liquid in hybridization chamber 2 via first and second agitation line 12,12′. This liquid yields somewhat toward third agitation line 12″, fills it, and flows back into first agitation chamber 11 via third agitation chamber...

third embodiment

[0040]FIG. 9 shows a specimen injection device having a two-part valve 49, during pipetting of the specimen liquid into specimen receiving vessel 42 (cf. FIG. 9A). A first part 50 of this two-part valve 49 is in its lowermost position and seals specimen supply line 7 of device 1. A preferably defined volume of the specimen liquid flows via pipette tip 51 into chamber 52 between specimen receiving vessel 42 and first valve part 50 via line 54, the surface of the liquid defining an appropriate level 53 and the excess air escaping via line 54′.

[0041]An excess pressure medium (e.g. nitrogen gas) is introduced into the space between first valve part 50 and annular second valve part 56 via connection 55, which causes second valve part 56, which has the excess pressure medium applied to it over a larger area, to yield downward (cf. FIG. 9B), until it touches the specimen liquid and is braked by it. At the same time, lines 54,54′ are automatically closed. The excess pressure, which is stil...

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Abstract

A device (1) is disclosed for providing a hybridization chamber (2), for hybridizing nucleic acid samples, proteins, or tissue sections on a slide (3), which is implemented so that it is movable in relation to this slide (3) and includes an annular sealing surface (4) for sealing the hybridization chamber (2) by being applied to a surface (5) of this slide (3), lines (6) for supplying and/or removing media to and/or from the hybridization chamber (2), and a specimen supply line (7). The devices according to the invention include a media-separating agitation device (8) for moving liquids in relation to samples immobilized on the surface (5) of the slide (3). Furthermore, process units (18) for hybridizing nucleic acid samples, proteins, or tissue sections and corresponding automatable systems including such process units (18) are disclosed.

Description

CROSS REFERENCES TO RELATED APPLICATIONS[0001]This application claims priority from Swiss Patent Application No. 0969 / 01, filed May 25, 2001 and Swiss Patent Application No. CH-0668 / 02, filed Apr. 22, 2002.FIELD OF THE INVENTION[0002]The present invention relates to a device as well as a corresponding process unit and an automatic system for hybridizing nucleic acid samples, proteins, and tissue sections.BACKGROUND OF THE INVENTION[0003]The use of DNA samples (DNA=deoxyribonucleic acid), and particularly microarrays of such samples, provides an important technology in research for the simultaneous analysis of thousands of genes. This technology includes the immobilization of DNA samples from many genes on a fixed substrate surface, for example on a glass slide for a light microscope. The DNA samples are preferably arranged in an array of sample spots or “spots”, i.e., in a two-dimensional grid on the substrate, and the origin of a corresponding DNA sample may be determined later bas...

Claims

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Application Information

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IPC IPC(8): B01L9/00B01L3/00B01F15/00B01F13/00B01L9/06B01F11/00B01F5/00B01F5/10G01N33/53C12M1/00C40B60/14G01N1/00G01N1/28G01N37/00
CPCB01F5/10B01F11/0071B01F13/0059B01F15/00123B01F15/00318B01F15/00396B01F15/0244B01L3/502715B01L3/50273B01L3/502738B01L3/50855B01L9/06B01F2015/00084B01F2215/0037B01F2215/0073B01J2219/00353B01J2219/00396B01J2219/00477B01J2219/00479B01J2219/00533B01J2219/00659B01L2200/0689B01L2300/0636B01L2300/0822B01L2300/0877B01L2400/0481C40B60/14B01F25/50B01F31/65B01F33/30B01F2035/351B01F35/20B01F35/2207B01F35/2215B01F35/71761B01F2101/23B01F2101/44
Inventor STREIT, WOLFGANGWENCZEL, GYOERGYMATHES, ANTONLAMPRECHT, WALTRAUD
Owner TECAN TRADING AG
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