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Method of processing and testing powdered samples using immunochromatographic strip tests

a strip test and immunochromatographic technology, applied in the field of immunochromatographic strip test methods, can solve the problems of increasing the cost of the test and time-to-result, affecting the quality of the test, and slow strip run, so as to achieve rapid, reliable and inexpensive results, simple, portable and user-friendly

Inactive Publication Date: 2008-04-08
STRATEGIC DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"The present invention provides a method and kit for analyzing powdered samples using an immunochromatographic test strip containing detectable reagents that are immunoreactive with an analyte to be detected in the sample. This method is rapid, reliable, and inexpensive, and can be utilized successfully by non-scientific personnel in the laboratory and the field. The results are accurate and reproducible, and the method provides a simple and portable way to detect analyte in a sample."

Problems solved by technology

However, very fine particulate matter suspended in the liquid sample clogs the pores of the strip test components causing the strip to run slowly, erratically or not at all.
Additional steps or test components employed to remove the particulates only serve to complicate the testing process and increase the cost of the test and time-to-result.

Method used

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  • Method of processing and testing powdered samples using immunochromatographic strip tests
  • Method of processing and testing powdered samples using immunochromatographic strip tests

Examples

Experimental program
Comparison scheme
Effect test

example 1

Genetically Modified Corn Sample Preparation and Analysis

[0033]This example illustrates the use of the method described herein to detect the Cry1Ab protein in a genetically modified, insect-resistant corn sample.

[0034]Each sample was weighed to obtain the proper number of corn kernels. Samples containing 60 kernels weighed 18.4 g and samples containing 125 kernels weighed 39.3 g.

[0035]Each sample was placed in a clean and dry glass, 4 oz. MASON™ jar.

[0036]A 70 mm blender base and blade assembly (Eberbach Co., Ann Arbor, Mich.) was attached. The MASON™ jar was placed onto the laboratory grade blender (Waring Model 31BL91) and covered with a safety shield. The sample was ground on high speed for 30 seconds to produce powdered corn. The safety shield, adapter and blade were removed.

[0037]The following specified amounts of buffer (Trait√™, also referred to as “Traitcheck”, sample buffer, Strategic Diagnostics Inc., Newark, Del.) was added to the powdered corn directly in the MASON™ jar....

example 2

Lateral Flow Test Kit and Procedure for the Detection of Cry1Ab Protein

[0043]This example a detailed procedure and kit to detect a Cry1Ab protein in a genetically modified, insect-resistant corn sample.

Kit Description

[0044]A kit is used to detect the Cry1Ab protein produced by a gene derived from Bacillus thuringiensis. This gene has been incorporated into insect-resistant corn including YIELDGARD™ brands from both Monsanto and Novartis. The intended uses of the kits include the qualitative (yes / no) determination of the Cry1Ab protein in plant leaf and seed tissue and corn grain samples. The lateral flow strips and other components provided in the kit are sufficient to make qualitative determinations for the presence of absence of the Cry1Ab protein in both field and laboratory environments. Different application protocols are required for leaf, seed and bulk grain determinations.

Principle of the Assay

[0045]The assay uses a double antibody sandwich format. Antibodies specific to the...

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Abstract

A method and kit for processing and testing powdered samples are provided herein. The powdered sample is combined with a fluid to produce a slurry or liquid suspension, and the slurry is contacted with an immunochromatographic test strip containing detectable reagents that are immunoreactive with an analyte to be detected in the sample. The method is particularly useful for the analysis of agricultural products or crops, such as the identification of recombinant grains.

Description

FIELD OF THE INVENTION[0001]This relates to the field of immunology and more specifically relates to a method for the analysis of a powdered sample.BACKGROUND OF THE INVENTION[0002]Many situations exist in which it is important to have rapid, reliable, inexpensive tests that can be run by untrained individuals in the field, home, or other non-laboratory setting. Immunoassays have many of these characteristics. One type of immunoassay, referred to as an immunochromatographic or lateral flow strip test, has been successfully developed into ‘one-step’ tests and employed for on-site analysis. The over-the-counter home pregnancy test is an example of a simple, one-step immunochromatographic strip test.[0003]Immunoassays have been used to detect substances in many different kinds of samples in many different markets, including the agriculture market. Modern biotechnology methods are being used to genetically modify plants. These genetically modified plants, and the seeds, grain and food d...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): G01N33/538G01N33/00G01N33/558G01N33/68
CPCG01N33/558G01N33/68G01N2033/0091Y10T436/25Y10S435/97Y10S435/805Y10S436/81Y10S436/807G01N33/0091G01N33/54388
Inventor KENNEDY, TARA ANNMCQUILLIN, ALAN BRUCE
Owner STRATEGIC DIAGNOSTICS