The invention relates to a
planet identification method based on a KASP marker. The
planet identification method comprises the following steps: (1) carrying out surface disinfection on a
plant sample to be tested; (2)
grinding the disinfected
plant leaves into
powder by using
liquid nitrogen; (3) extracting the
genome DNA of the sample, adjusting the
DNA concentration to 5-50ng / mu L, and storing at 4 DEG C for later use; (4) selecting a specific SNP (
Single Nucleotide Polymorphism) site on the basis of comparing the ppc-1E1c sequences of the astrophe plants; (5) designing two forward primers and a
reverse primer according to the SNP site; (6) respectively adjusting the concentrations of the three primers to 36 [mu] M, 36 [mu] M and 90 [mu] M, and mixing the three primers according to a volume ratio of 1: 1: 1 to form a primer
mixed solution; (7) preparing a reaction solution according to a
reaction system: 5 [mu] L of template
DNA, 5 [mu] L of KASP mastermix and 0.14 [mu] L of the primer
mixed solution, and setting a
negative control; (8) performing amplification according to a PCR program; and (9) judging whether the sample is a
planet plant or not according to a
fluorescence typing result. The method is rapid, accurate, low in cost and suitable for
species identification,
germplasm resource identification and
molecular marker-assisted breeding of planets.