The invention provides a biological compound preparation for preventing and controlling nematodes as well as a preparation method and application of the biological compound preparation. The biological compound preparation is prepared from the following components in parts by weight: 50 to 90 parts of
oligosaccharide extracting solution, 15 to 35 parts of first
plant extract and 15 to 35 parts of second
plant extract, the first
plant extract comprises any one or a combination of at least two of a
pyrethrum extract, a garlic extract, a
turmeric extract or a
lavender extract; the second plant extract comprises any one or a combination of at least two of a
marigold extract, a
mugwort extract, a
derris extract or a
mint extract. The biological compound preparation prepared by utilizing the principle of natural biological defense lines in the nature has an excellent
nematode control effect, the components are safe and
pollution-free, and
drug resistance is not generated; meanwhile, required nutritional ingredients are further provided for the plants, the growth of the plants is promoted, and the
disease resistance of the plants is improved.