This application discloses a liver
peptide with liver-protective and eye-fatigue-relieving properties, its preparation method, and its applications, belonging to the field of
biotechnology. The liver
peptide contains at least one of DAIP, SPF, DIAP, SLP, AVGP, or NLP. The prepared liver
peptide has multiple physiological functions: it can effectively activate ADH
enzyme activity, reduce ALT and AST levels, and protect hepatocytes; it improves
lipid metabolism by regulating the expression of SREBP-1c, FAS, and PPAR-α genes; it also has significant
antioxidant capacity, reducing ROS levels and increasing SOD and CAT content; and it can upregulate RPE65 and LRAT
gene expression, maintaining
visual function. The method disclosed in this invention is simple and convenient to operate, and the obtained liver peptide can be widely used in functional products for liver protection and
eye fatigue relief, providing an innovative solution for the high-value utilization of liver resources.