Application of ursane type triterpenoid saponin in the preparing process of leucocyte and/or platelet increasing medicine
A technology of ursane-type and triterpene saponins, which is applied in the field of compounds and can solve problems such as increasing the activity of human white blood cells and platelets
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Embodiment 1
[0032] Example 1: Extraction, separation and structure confirmation of ursane-type triterpene saponins A, B, C
[0033] Extraction and separation: Sanguisorba officinalis L. was purchased from Chengdu Traditional Chinese Medicine Company. Take 20kg of Sanguisorba medicinal material, slice it, extract twice with 8 times 70% ethanol, filter for 1 hour each time, recover the ethanol, add water to 0.3g crude drug / ml, refrigerate for 12 hours, take the supernatant and absorb through the macropores Resin. First wash with water until the Molish reaction is negative, then eluted with 70% ethanol, collect the eluate, recover the ethanol, and concentrate to obtain a concentrated extract. Add 25L of hot water to the concentrated extract and stir thoroughly, and then suction and filter out the precipitate (about 4kg, mainly tannin detected by TLC). The filtrate was extracted three times with equal volumes of ethyl acetate and n-butanol, and then concentrated under reduced pressure and evapora...
Embodiment 2
[0038] Example 2: Therapeutic effects of ursane-type triterpene saponins A, B, C, D, E, F, G, H on leukopenia and thrombocytopenia induced by cyclophosphamide in mice
[0039] Materials and Methods
[0040] Test drug: The three samples of compound A, B, and C are all off-white powders or broken crystals, prepared according to the method of Example 1, and can also be separated, identified and prepared with reference to the literature [Triterpene glycosides from the roots Sanguisorba officindis, Phytochenistry, 57(5), 773-779, 2001]; Compound D, E, F refer to literature [Triterpene glycosides from the roots Sanguisorbaofficindis, Phytochenistry, 57(5), 773-779, 2001] method of separation, identification and preparation. Compound G was isolated, identified and prepared with reference to the literature [phytochemistry, 26(7), 2023, 1987; Chen.pharm. Bull. 35(2), 524, 1987]. Compound H was isolated, identified and prepared with reference to the literature [phytochemistry, 21(6), 1373, ...
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