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Culturing method of wilsonii withont adding plant hormone

A plant hormone and culture method technology, applied in the field of Acanthopanax senticosus cultivation, can solve the problems of plant hormone harmful to human body, low germination rate, low rooting rate of cuttings, etc., and achieve the effect of simple technology, high proliferation rate and fast germination speed

Inactive Publication Date: 2008-10-08
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problems that the current artificially cultivated seeds are difficult to deal with, the germination rate is low, the rooting rate of cuttings is low, and the plant hormones used in the existing cell culture technology are harmful to the human body, and a kind of stimulant without adding plant hormones is provided. Five plus culture method
[0004] The invention avoids the problems of difficult seed treatment, low germination rate, and low rooting rate of cuttings in artificial cultivation, does not add any plant hormones, is safe and reliable, and has simple technology, fast germination speed, high multiplication rate, and secondary embryo occurrence rate of up to 100%. 100%

Method used

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  • Culturing method of wilsonii withont adding plant hormone
  • Culturing method of wilsonii withont adding plant hormone
  • Culturing method of wilsonii withont adding plant hormone

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specific Embodiment approach 1

[0006] Specific embodiment one: the Acanthopanax cultivating method that does not add phytohormone in this embodiment is carried out according to the following steps: (1) the seed of Acanthopanax and coarse sand are mixed by the volume ratio of 1: 10, put into 15 ℃ of cultivating Stratify in the box for 3 to 4 months, water once a week to keep the coarse sand moist; (2) remove the outer testa of the mature seeds in embryo form, and put the seeds with the removed outer testa into 1% sodium hypochlorite Soak in the solution for 4-10 minutes, then inoculate in medium without adding plant hormones, place at 25±1°C, light intensity 25-45μmol / m 2 s. Cultivate in an environment where the sunshine time is 14-16 hours; (3) select the seeds whose cotyledons come out first, and when the cotyledons are exposed outside the endosperm for 1-3 days, they will be transferred to an environment with a temperature of 37-42°C for high-temperature stress treatment for 1-3 days. 5 days; (4) Put the ...

specific Embodiment approach 2

[0008] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the culture medium that is used for inoculation in step (two) is to add 10~90g sucrose and 5.5g agar per liter, pH value is 5.7~5.8, inorganic The MS medium whose salt concentration is 1 / 3~1 / 2 of the original concentration. Other steps are the same as those in Embodiment 1.

specific Embodiment approach 3

[0009] Specific embodiment three: the difference between this embodiment and specific embodiment one is: Step (3) Repeat the high temperature stress treatment on the seeds that have not stopped growing after the high temperature stress treatment, until the seeds stop growing. Other steps are the same as those in Embodiment 1.

[0010] This embodiment is carried out 1 to 2 times, and the cotyledon first-out seeds all stop developing, and more seeds capable of growing somatic embryos can be obtained.

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Abstract

A method for culturing the acanthopanax without plant hormone includes such steps as laminar stacking of seeds at 15 deg.C, culturing the peeled seeds, high-temp stressing, continuous culturing, culturing the fragment of the young plant developed from the somatic embryo or the somatic embryo, culturing secondary embryo, and culturing young plant. It has high germination speed, reproduction rate and the generation rate of secondary embryo (100%).

Description

technical field [0001] The invention relates to a method for cultivating Acanthopanax senticosus. technical background [0002] Eleutherococcus senticosus Maxim is a perennial deciduous shrub of Araliaceae. Its roots, stems, and leaves can be used as medicine. Because of its remarkable medicinal value, the international market price of its extract is much higher than that of ginseng. With the depletion of wild resources and the increase of market demand, the artificial cultivation technology of Acanthopanax is paid more attention. However, the current artificial cultivation of Acanthopanax has the problems of difficult seed treatment, low germination rate and low rooting rate of cuttings; although the existing cell culture technology can make up for the above defects, the phytohormones used in the cell culture process have been proved to be harmful. Harmful to the human body (2,4 dichlorophenoxyacetic acid, etc. have carcinogenic effect), so the safety of eleuthero senticos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 李成浩杨传平刘桂丰牛遇达赵波韩君
Owner NORTHEAST FORESTRY UNIVERSITY