Artificial feeding milk for sterilized rat

Abstract

The present invention discloses germ-free rat artificial feeding milk. According to the postnatal age in days of suckling mouse it can be divided into 0-10-day group and 11-21-day group. Said artificial feeding milk for germ-free rat is formed from raw materials of rabbit milk, cow milk, rat serum, lactose, amino acids, vitamins, milk powder, egg and protein powder, etc.

Description

technical field [0001] The invention relates to a milk product for artificial feeding of animals, in particular to a sterile milk for artificial feeding of rats. Background technique [0002] Artificial milk for sterile rats refers to the artificial milk used for artificial lactation of newborn mice obtained by caesarean section under sterile conditions. Rat milk is difficult to obtain, and milk fed to newborn rats must be autoclaved to ensure sterility. However, high-pressure sterilization greatly destroys nutrients, especially vitamins, so it is necessary to select suitable animals as milk sources and supplement the destroyed components. [0003] There are currently 4 kinds of artificially fed milk for rats that have been published, and the specific composition is shown in Table 1: [0004] Table 1 [0005] [0006] [0007] The above-mentioned 4 kinds of formulas were tested, and the following problems were found after the test: [0008] 1. Formula 1 and formula...

AI Technical Summary

Problems solved by technology

[0008] 1. Formula 1 and formula 2, the ingredients are too complicated, some ingredients are not necessary, the milk used is milk or milk powder, so the protein content is low; and the artificial milk is relatively thin after autoclaving, and it is only suitable for forced feeding Hello, not for the automatic sucking method

Because suckling rats sucking thin milk can easily inhale the milk into the lungs and cause foreign body pneumonia, resulting in growth retardation or death of suckling rats;

[0009] 2. The protein content of formula 3 is higher than that of formula 1 and 2, but the ingredients are still very complex. After autoclaving, the artificial milk is still very thin, and it is necessary to add sterilized milk powder to increase its viscosity

During use, it was found that the milk formulated with this formula coagulated after high-pressure sterilization, and it took a long time to grind with a tissue grinder to form a relatively uniform liquid, but there were still some small particles, which affected digestion and absorption

Moreover, grinding artificial milk with a grinder is also a big loss, and water must be added when grinding, which is difficult to quantify.

In addition, in our experiments, we found that when feeding suckling mice with this kind of milk, large curds as hard as stones can be formed in the stomachs of some suckling mice, and the curds account for more than 50% of the stomach capacity. Suckling and emptying of milk by suckling mice, which in turn affects the growth and development of suckling mice

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