Mouse asthmatic model semiotic monitor

Abstract

A physical signs monitor with acoustic sensor for the asthma model of mouse features that a medical stethoscope is used as the sensing element to acquire the cardiac pulse wave of the mouse when it has an asthma attack for quantitatively analyzing the variation of its physical signs. Said monitor is composed of asthma exciter, mouse to be tested, asthma sign observing cabin, acoustic sensor, computer and sound wave amplifier.

Description

technical field [0001] The experimental method relates to the technical field of monitoring the signs of an experimental animal model of asthma. Background technique [0002] Asthma is a ubiquitous allergic disease, especially in western developed countries, its incidence rate is as high as 5-30%, and the incidence rate of children can reach 50%. Asthma has become an important high-incidence disease that seriously affects human health in today's society. The pathogenesis of asthma is complex and has not yet been fully elucidated. The construction of animal models of asthma and its monitoring methods are important methods for asthma research. Because the immune genetic background of mice is clearer than that of other animals, there are more relevant molecular biology reagents and antibodies available for selection, and its pathogenesis is close to that of humans (IgE-mediated type I hypersensitivity reaction), which can be explored using mouse asthma models Etiology and path...

AI Technical Summary

Problems solved by technology

However, due to the small size and high respiratory rate of mice, it is difficult to observe the wheezing sound that can be perceived by human hearing. The traditional signs observation indicators are subjective indicators, such as shortness of breath, cough, irritability and even shock. Quantitative monitoring of asthma is very difficult, so there is no feasible quantitative index for the identification of asthma attack and the detection of the strengthening or weakening effect of drugs on asthma signs

At present, only the OVA-sensitized mouse asthma model can be used at home and abroad to detect and analyze specific antibodies, cytokines, and inflammatory cells. For the analysis of pathological signs of the disease, more subjective analysis indicators such as onset latency, sign index grading and scoring are mostly used. , poor experimental accuracy and repeatability

Because mice are small and have narrow airways, it is difficult to design and manufacture a device for measuring lung function in mice. At present, there are no breathing and heartbeat monitoring instruments for mice in China. The latest physiological monitoring instruments for mice abroad are very expensive. And it is difficult to meet the needs of asthma model monitoring

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