Fluorescence PII sensor with inner reference and comparison, and preparation method
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A ph sensor and internal reference technology, applied in the field of sensors and their preparation, to achieve the effects of good reproducibility and reversibility, short response time, and prevention of loss
Inactive Publication Date: 2009-08-19
HUNAN UNIV
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However, there are few such pH-sensitive fluorescent dyes with dual wavelengths at present; the other is to use two fluorescent dyes, namely, a sensitive dye that is sensitive to pH changes and a reference dye that is insensitive to pH changes to achieve a fluorescence ratio. Measurement
Although there are many kinds of known pH-sensitive dyes, most of the reported literatures are limited to fluorescein derivatives as sensitive dyes.
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Embodiment 1
[0024] 1. Preparation of fluorescent pH sensor with internal reference 1:
[0025] a. Synthesis of sensitive dye porphyrin derivative TAPP: 1.0g 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin (synthetic method reference document: Chinese Science B Series, June 1997, Volume 27 Phase 3) dissolved in 20ml N,N-dimethylformamide (12.98mol l -1 ), add 2g potassium carbonate, 2ml bromopropene (11.56mol l -1 ), reacted at 100°C for 4 hours, cooled, the reactant was poured into 30ml of distilled water, and crystals were precipitated. The crude product was chromatographed on a neutral alumina column (100-200 mesh) with chloroform as the eluent, and the product TAPP was obtained as a purple solid.
[0026] b. Synthesis of the reference dye benzothioxanthene derivative MBTD: N-hydroxyethyl-benzothioxanthene-3,4-dicarboximide (synthetic method reference document: J.Heterocyclic Chem.1974 , 11,33) 0.60g was dissolved in 70ml of dried N,N-dimethylformamide (the drying method was reflux dryi...
Embodiment 2
[0039] 1. Preparation of fluorescent pH sensor with internal reference 2:
[0040] a. Synthesize the sensitive dye TAPP and the reference dye MBTD according to the method described in step 1 of Example 1.
[0041] b. Silanize the surface of the glass slide according to the method described in Step 1 of Example 1.
[0042] c.TAPP15mg and MBTD1.5mg were dissolved in 0.40ml N,N-dimethylformamide (12.98mol l -1 ), followed by adding 250mg acrylamide, 0.40ml hydroxyethyl methacrylate (8.24mol l -1 ), 0.15ml crosslinking agent triethoxytrimethacrylate (3.78mol l -1 ), 0.20ml triethanolamine (7.54mol l -1 ), 0.25ml photosensitizer 2-hydroxyl-methylphenylpropane-1-one (6.58mol l -1 ).
[0043] d. Take 0.20ml of the above-mentioned solution and drop it on a clean polytetrafluoroethylene plate, cover it with a silanized glass slide, then irradiate it with an ultraviolet lamp (254nm) for 25min, rinse it with distilled water, and dry it at room temperature to obtain A glass slide co...
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Abstract
A fluorescent pH sensor with an internal reference includes a silanized glass slide, and porphyrin derivatives and benzothioxanthene derivatives. A method for preparing the fluorescent pH sensor with an internal reference comprises the following steps: dissolving porphyrin derivatives and benzothioxanthene derivatives in N,N-dimethylformamide, adding acrylamide, formaldehyde Hydroxyethyl acrylate, triethylene glycol dimethacrylate, triethanolamine and 2-hydroxy-methylphenylpropan-1-one, take an appropriate amount of the final solution and drop it on a polytetrafluoroethylene plate, and use silanization A glass slide is covered thereon, irradiated with ultraviolet light, rinsed with distilled water after photopolymerization, and dried at room temperature, and a fluorescent pH sensor with an internal reference is prepared. The invention has the advantages of realizing the preparation of the fluorescence pH sensor covalently immobilizing the sensitive dye and the reference dye at the same time, and the measurement signal can more accurately reflect the change of pH.
Description
technical field [0001] The invention relates to a sensor and a preparation method thereof, in particular to a fluorescent pH sensor with an internal reference and a preparation method thereof. Background technique [0002] The determination of pH value is very important in various scientific researches and practical applications, and the development of pH sensors has also attracted much attention. At present, the most widely used pH sensor is the glass electrode pH sensor, but it has high impedance, is easy to be damaged, and has defects such as "sodium error" in the case of high alkalinity. [0003] The optical fiber pH sensor has greatly improved the deficiency of the glass electrode pH sensor. In the research of the optical fiber pH sensor, due to the inherent sensitivity and selectivity of the fluorescence method, the richness of the fluorescence measurement signal and the simplicity of the design, the sensor based on the fluorescence principle Research is extremely act...
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