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Preparation method of high magnetism content monodisperse hydrophilicity magnetic composite microsphere

A technology of magnetic composite microspheres and magnetic microspheres, which is applied in the field of biological separation materials, can solve the problems of inhomogeneity, low content of magnetic particles, and low magnetic content of microspheres, and achieve controllable thickness, simple and feasible operation, and good hydrophilicity performance effect

Active Publication Date: 2009-10-28
SHANGHAI ALLRUN NANO SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the hydrophilic magnetic microspheres have been obtained in this paper, the disadvantage is that the magnetic content of the synthesized microspheres is very low. The core of each magnetic ball is composed of a magnetic particle, so the magnetic response of the microspheres is very low. Low, unable to meet the needs of downstream biomedical applications
At present, the use of silicon oxide-coated superparamagnetic materials has the problem that the content of magnetic particles in the microspheres (or materials) is low or not superparamagnetic, or the particle size of the microspheres is not uniform, which limits their application.

Method used

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  • Preparation method of high magnetism content monodisperse hydrophilicity magnetic composite microsphere

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Using the miniemulsion-emulsion polymerization method to obtain monodisperse magnetic microspheres with high magnetic content as the base core to prepare monodisperse hydrophilic magnetic microspheres with high magnetic content

[0020] 1. Fe 3 o 4 Preparation, surface modification and Fe 3 o 4 Preparation of octane dispersion

[0021] 24gFe 3 Cl.7H 2 O and 18gFe 2 SO 4 .7H 2 Dissolve O in 100ml of deionized water, pour it into a three-neck flask after fully dissolving, and place it in a water bath at 80°C, fill it with nitrogen, stir at 200rpm for half an hour, then add 50g of ammonia water into the reaction system to start the reaction. After half an hour 3.5 g of oleic acid was added. After one hour, the reaction system was opened, and the reaction was terminated after the ammonia water was completely evaporated. With the assistance of a magnetic field, it was fully washed with deionized water until the supernatant was neutral, then washed three t...

Embodiment 2

[0026] Embodiment 2 utilizes surfactants such as Tween in nanometer Fe 3 o 4 Self-assembly of particle surface to obtain high magnetic content monodisperse magnetic particle spherical aggregates as the base core to prepare high magnetic content monodisperse hydrophilic magnetic microsphere

[0027] 1. Fe 3 o 4 Preparation, surface modification and Fe 3 o 4 Preparation of octane dispersion

[0028] Fe 3 o 4 The preparation of magnetic nanoparticles is the same as described in Example 1-1. The difference is that the amount of oleic acid is 2 grams, and finally it is made into Fe with a magnetic content of 50wt%. 3 o 4 Octane dispersion.

[0029] 2. Using Tween and other surfactants in nano-Fe 3 o 4 Self-assembly of particle surface to obtain monodisperse magnetic particle spherical aggregate base core with high magnetic content

[0030] First, the above-mentioned 5 grams of nano-Fe 3 o 4 Add the octane dispersion into 150ml of Tween 20 aqueous solution with a conc...

Embodiment 3

[0034] Nano Fe3O4 particle preparation method is as described in Example 1, the difference is the preparation of nano Fe 3 o 4 Fe used 2+ Be ferrous chloride, and consumption is 10 grams, and used oleic acid is 5 grams, and the magnetic content of preparation is the Fe of 60wt%. 3 o 4 Octane dispersion.

[0035] The preparation method of high magnetic content monodisperse magnetic-based core is as described in Example 1, the difference is that the microemulsion A with an average particle diameter of 176nm is prepared by 200W ultrasound, and the average particle diameter is 6000nm after being stirred at 300rpm for 0.5 hours. For miniemulsion B, the polymerization reaction temperature is 60°C, and high magnetic content monodisperse composite magnetic microspheres with an average diameter of 198nm are obtained.

[0036] The preparation of monodisperse hydrophilic magnetic composite microspheres with high magnetic content is as described in Example 1, the different dosages of ...

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Abstract

The invention discloses a method for preparing monodisperse hydrophilic magnetic composite microspheres with high magnetic content, which belongs to the field of biological separation materials. The present invention first adopts co-precipitation method to prepare oleic acid-modified nano ferric oxide particles, and then prepares spherical aggregates or microspheres of monodisperse magnetic particles with high magnetic content, that is, adopts a method based on miniemulsion-emulsion polymerization to obtain high magnetic content. Monodisperse magnetic microspheres, using Tween to self-assemble on the surface of nano-magnetic particles to obtain monodisperse magnetic particle spherical aggregates with high magnetic content, and then use the above two magnetic microspheres or magnetic spherical aggregates as hydrophilic magnetic microspheres The base core was coated with a silica shell by using the sol-gel method to finally obtain monodisperse hydrophilic silica magnetic microspheres with high magnetic content. While ensuring that the finally obtained microspheres have good hydrophilic properties, the present invention greatly improves the content of magnetic substances in the obtained hydrophilic nano magnetic microspheres and the uniformity of the size and shape of the microspheres.

Description

technical field [0001] The invention relates to a method in the technical field of biological separation materials, in particular to a method for preparing monodisperse hydrophilic magnetic composite microspheres with high magnetic content. technical background [0002] In recent years, nano-superparamagnetic particles or microspheres have been widely used in the fields of biology and medicine due to their excellent characteristics. Generally, magnetic carriers used in the field of biological separation and purification should have the following characteristics: superparamagnetic, uniform particle or microsphere size, surface functional groups, fast magnetic response characteristics (high and uniform magnetic substance content) , no leakage of magnetic materials and good biocompatibility, etc. [0003] As the most important magnetic carrier, the study of magnetic microspheres began in the late 1970s. Ugelstad successfully prepared monodisperse magnetic polystyrene microsphe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F2/04C08K3/20
Inventor 徐宏崔陇兰童乃虎古宏晨侯丽英查全亮
Owner SHANGHAI ALLRUN NANO SCI & TECH