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Active constituent of curcuma longa for inhibiting and killing plant pathogenic fungi and its preparation and application

A technology of phytopathogenic fungi and active ingredients, which is applied in the field of plant pesticides and can solve the problems of phytopathogenic fungi causing great harm to plants

Inactive Publication Date: 2009-12-23
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, almost all of the botanical pesticides registered today are insecticides, and there are very few preparations that inhibit and kill phytopathogenic fungi. In agricultural production, phytopathogenic fungi are extremely harmful to plants, which is an obstacle to increasing agricultural production and farmers' income. Therefore, there is an urgent need to provide more varieties of pesticide preparation products that can effectively inhibit and kill plant pathogenic fungi, are environmentally friendly and can ensure the safety of humans and animals, so as to increase farmers' income

Method used

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  • Active constituent of curcuma longa for inhibiting and killing plant pathogenic fungi and its preparation and application
  • Active constituent of curcuma longa for inhibiting and killing plant pathogenic fungi and its preparation and application
  • Active constituent of curcuma longa for inhibiting and killing plant pathogenic fungi and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Preparation and antibacterial activity assay of embodiment 1 turmeric extract

[0018] Steps to prepare turmeric extract:

[0019] (1) The dried turmeric rhizome (or tuber) is crushed and passed through a 40-mesh sieve to obtain turmeric dry powder.

[0020] (2) Take 95% ethanol equivalent to about 7 times the volume of the raw material (the general control range is 5 to 10 times) and extract it at room temperature (the temperature is generally in the range of 18 to 24 ° C) for about 20 hours (the general control range is 12 ~24h).

[0021] (3) Continuous stirring and extraction in a constant temperature water bath at about 50°C (general control range is 45-60°C) for about 10h (general control range is 6-12h). Use a Buchner funnel to filter under negative pressure to remove the residue to obtain the filtrate ①.

[0022] (4) The residue is further agitated and extracted for 8 hours (the general control range is 6 to 12 hours) with 95% ethanol equivalent to about 6 tim...

Embodiment 2

[0034] The influence of embodiment 2 turmeric ethanol total extract, n-hexane extract on germination of pathogenic bacteria spores

[0035] (1) Bacteria to be tested: wasabi spp., olive green mold, Penicillium pallidum, Verticillium spp.

[0036] (2) Adopt the concave slide method: take the prepared drug-containing spore suspension (the final concentration of the drug solution is 10mg·ml -1) and 100 μl of the spore suspensions of the blank control and the acetone control were dropped on the concave glass slide, put into a sterilized petri dish, and placed in a 26°C incubator for cultivation. Four replicates were set for the liquid treatment and the control group. . Check for spore germination after 12 hours. Microscopically inspect 10 fields of view, observe about 200 spores in each treatment, and calculate the spore germination rate. When the spore germ tube length exceeds half of the spore diameter length (minimum diameter), as the spore that has germinated, the calculati...

Embodiment 3

[0043] Example 3 Preparation and bioactivity determination of turmeric n-hexane extract.

[0044] (1) The total ethanol extract of turmeric was extracted and separated with 8 times the volume of n-hexane to obtain n-hexane extract, and the extract was concentrated with a rotary evaporator at a concentration temperature of about 40°C to obtain a paste-like extract.

[0045] (2) The above-mentioned extraction paste is configured with acetone solution to a concentration of 0.05g ml -1 The medicinal solution was used as a spare sample for the experiment. Using wasabi sclerotium, rape sclerotium, tomato cinerea, verticillium gibberella, wheat gibberella, edible fungus verruca, olive green mold, and penicillium pallidum as the test bacteria, the determination of the concentration of Antibacterial activity, antibacterial activity assay method is the same as embodiment 1.

[0046] The experimental results are shown in Table 3.

[0047] Table 3 Inhibitory effect of turmeric n-hexane...

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Abstract

The invention discloses an active component of turmeric for inhibiting and killing plant pathogenic fungi, as well as its preparation and application. The active ingredient is the extract obtained by extracting turmeric with ethanol or methanol as the extraction solvent, or the fine extract obtained by further extracting with n-hexane as the extraction solvent on the basis of the extract obtained by using ethanol or methanol as the extraction solvent. The active ingredient of turmeric that inhibits and kills phytopathogenic fungi can be used to prepare preparations for inhibiting and killing phytopathogenic fungi and mushroom pathogenic fungi, especially can be used to prepare anti-killing horseradish ink bacteria, rapeseed sclerotinia, tomato cinerea, verticillium Preparations of sporozoites, gibberella tritici, edible fungus verruca, olive green mold or penicillium pallidum etc., the formulations can be in the form of water, soluble powder, wettable powder, suspension and granules. The turmeric active ingredient disclosed by the invention has the characteristics of wide source of production raw materials, non-toxic to human body, harmless to crops, easy to degrade, etc., and can be developed into a pollution-free botanical antifungal preparation.

Description

technical field [0001] The present invention relates to plant pesticide technology, more specifically, relates to a plant extract active ingredient for inhibiting and killing plant pathogenic fungi, its preparation method and application. Background technique [0002] Before the use of chemically synthesized pesticides, many of the earliest pesticides were extracted from plants, and the natural active substances of some plants have been developed into commercial pesticides, such as rotenone and nicotine. However, since the 1940s, organic chemical synthetic pesticides have replaced botanical pesticides and dominated the market, causing the research on botanical pesticides to fall into a slump. At present, more than 80% of the pesticides used in China are chemical preparations. These chemical pesticides have played an important role in reducing plant diseases and insect pests and increasing crop yields, but at the same time they have also brought about environmental pollution,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/00A01P3/00A01N65/48
Inventor 龙章富尉研崔佳刘世贵王立洪
Owner SICHUAN UNIV