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Protein forage and preparing method thereof

A protein feed, protease technology, applied in the field of microbial engineering, can solve the problems of affecting safety, easy to produce salmonella, anti-nutritional factors and toxicity problems

Inactive Publication Date: 2009-12-23
NANTONG HONGTONG BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Both meat and bone meal and fish meal are animal protein, which often have anti-nutritional factors and toxicity problems, which affect the safety of use; moreover, salmonella is easily produced in animal protein feed, which is very harmful to humans and animals
Plant protein also has anti-nutritional factors and pollution caused by incomplete absorption

Method used

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  • Protein forage and preparing method thereof
  • Protein forage and preparing method thereof
  • Protein forage and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] 1) Activate Bacillus subtilis (CICC10265) at 28°C, culture in liquid, culture in solid, and dry to obtain 6 kg of bacterial powder;

[0101] 2) Activate Lactobacillus plantarum (CICC6001) at 30°C, culture in liquid, culture in solid, and dry to obtain 5.5 kg of bacterial powder;

[0102] 3) Add 80 kg of molasses to 100 kg of water, heat and dissolve at 60°C, cool the obtained solution to 30°C, add the bacteria powder in 1) and 2), mix, activate, and mix with 2kg of protease (Proteinase), After mixing evenly, add 1.0 tons of soybean meal, solid-state fermentation for 2 days, and dry with hot air to obtain the final product.

Embodiment 2

[0104] 1) Activate Bacillus subtilis (CICC10075) at 30°C, culture in liquid (shaking table), culture in solid, and dry to obtain 2 kg of bacterial powder;

[0105] 2) Activate Lactobacillus brevis (CICC6004) at 37°C, and obtain 10L of bacterial liquid after static fermentation;

[0106] 3) Add 20 kg of molasses to 100 kg of water, heat and dissolve at 60°C, cool the obtained solution to 30°C, add the bacterial powder and bacterial liquid in 1) and 2), mix, activate, and mix with protease (Proteinase) 3kg was mixed, and after mixing evenly, 1.0 tons of soybean meal was added, solid-state fermentation was carried out for 5 days, and the final product was obtained through hot air drying.

Embodiment 3

[0108] 1) Activate Bacillus megaterium (ACCC10008) at 28°C, culture in liquid, culture in solid, and dry to obtain 10 kg of bacterial powder;

[0109] 2) Activate Saccharomyces cerevisiae (CICC1856) at 28°C, and obtain 7kg of bacterial powder through liquid culture, solid culture and drying;

[0110] 3) Lactobacillus bulgaricus (CICC6045), Streptococcus lactis (CICC60036), and Lactobacillus plantarum (CICC6001) were respectively activated at 30°C for primary liquid culture, and then mixed for secondary culture to obtain 16 kg of bacterial liquid;

[0111] 4) Add 150 kg of molasses to 100 kg of water, heat and dissolve at 70°C, cool the obtained solution to 30°C, add the bacterial powder in 1), 2) and the bacterial liquid in 3), mix and activate, add 1.0 tons of soybean meal, solid-state fermentation for 4 days, and drying with hot air to obtain the final product.

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PUM

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Abstract

A protein feed rich in lactic acid, micropeptide and amino acids is prepared from soybean cake through biological predigestion. It can decrease the phosphorus pollution caused by phosphorus phytate contained in vegetative feed, and increase the utilization rate of protein.

Description

Technical field: [0001] The invention relates to a protein feed and a preparation method thereof, belonging to the field of microbial engineering. Background technique: [0002] The feed industry is directly related to the national economy and people's livelihood. According to data, China's feed consumption in 2000 reached 65 million tons. In feed production, the demand for soybean meal is increasing rapidly. [0003] Animal nutrition studies have shown that simply adding soybean meal as protein to feed pigs and chickens is often not fully utilized, and excess ammonia and nitrogen are excreted from the feces of poultry and livestock, causing damage to water, soil and air. pollution, this situation is called nutrient pollution. [0004] Studies have found that most of the digested protein is absorbed as oligopeptides rather than monomeric amino acids, and various peptides are transferred to animal cells much faster than free amino acids. Therefore, in order to further imp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/14A23K10/12A23K10/37
CPCY02P60/87
Inventor 顾建洪
Owner NANTONG HONGTONG BIO TECH
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