Human papillomavirus detection and parting reagent kit
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A technology of human papillomavirus and kit, which is applied in the detection/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., and can solve problems such as little clinical value
Active Publication Date: 2010-01-20
SHANGHAI TELLGEN LIFE SCI CO LTD
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Problems solved by technology
According to clinical needs, these main types should be distinguished, so the corresponding probes must be strictly distinguished; for other rare types, especially low-risk types, the clinical value of distinguishing each type is not great
Method used
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Embodiment 1
[0094] Probe one-to-one coating method
[0095] 1. Preparation of chips for detecting HPV6, HPV11, HPV16 and HPV18:
[0096] 1. Synthesize oligonucleotide probes according to the following sequence
[0097] name
sequence
SEQ ID
NO
HPV06
5′NH 2 TTTTTTTTTTAGACGTTCGATTTCCACTAC3′
01
HPV11
5′NH 2 TTTTTTTTTTGGGGGTAATAACAGATCATC3′
02
HPV16
5′NH 2 TTTTTTTTTTACCTCTGATGCCCAAATA3′
09
HPV18
5′NH 2 TTTTTTTTTTCAGGTATGCCTGCTTCAC3′
11
Globin
5′NH 2 TTTTTTTTTTCCCTGACTTTTTATGCCCAG-3′
53
[0098] (positive control)
NC
(negative control)
5′NH 2 TTTTTTTTTTCGTTAGGTCGGAGCTTGATC-3′
54
[0099] 2. Select 6 kinds of microspheres No. 31, 32, 33, 34, 35, and No. 36 [Luminex Company] to coat the probe according to the following method:
[0100] (1) Put six types of microspheres No. 31, 32, 33, 34, 35, and No. 36 and a ...
Embodiment 2
[0200] Probe mixed coating method
[0201] 1. Preparation of chips for detecting HPV6, HPV11, HPV53, HPV54, HPV40, HPV42, HPV43, HPV44, HPV16, HPV18, HPV31, HPV33, HPV35, HPV39, HPV45, HPV51, HPV52, HPV56, HPV59, HPV58 and HPV66:
[0202] 1. Synthesize oligonucleotide probes according to the following sequence
[0203] HPV06
5′NH 2 TTTTTTTTTTAGACGTTCGATTTCCACTAC 3′
HPV11
5′NH 2 TTTTTTTTTTGGGGGTAATAACAGATCATC 3′
HPV53
5′NH 2 TTTTTTTTTTTTTCTCACCAATAACGC 3′
HPV54
5′NH 2 TTTTTTTTTTGGAGTTGCAGCATAAATAGA 3′
HPV40
5′NH 2 TTTTTTTTTTCAATAGGAGTCCGACCAG 3′
HPV42
5′NH 2 TTTTTTTTTTGGCAGACATAATTTAGGTAGTA 3′
HPV43
5′NH 2 TTTTTTTTTTTCTAATACCAGGTCCAAAAT 3′
[0204] HPV44
5′NH 2 TTTTTTTTTTGCACTTTTAATAACCAGATCCT3′
HPV16
5′NH 2 TTTTTTTTTTGAAAATAATTTGAACTGGCT 3′
HPV16
5′NH 2 TTTTTTTTTTACCTCTGATGCCCAAATA 3′
HPV18
5′NH 2 TTTTTTTTTTCAGGTATGCCTGCTTCAC...
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Abstract
A testing and typing method and its reagent boxes for Human Papillomavirus(HPV), relating to exciters and nucleic acid probes which are especially for testing and typing of HPV. The invention fixes three types of special probes including different subgroups of low-hazard HPV and high-hazard HPV, subgroups with extra-low infection rate of low-hazard or high-hazard HPV, onto the same position or mediator. The invention also fixes oligomeric nucleoside probes of special HPV onto microballoons based on Luminex xMAP technical platform, which forms microballoons probes for testing HPV subgroups and typing slugs of HPV. A testing and typing method with simplicity of operator and its reagent boxes are invented. The method has much strongpoint including high-sensitivity, high-flux, stable result and good repeatability.
Description
technical field [0001] The invention relates to a nucleic acid probe, in particular to a nucleic acid probe for detection and typing of human papillomavirus (HPV) and a kit thereof. Background technique [0002] Human papillomavirus (Human papillomavirus, HPV) is an epitheliophilic virus, which is widely distributed in humans and animals and has a high degree of specificity. For a long time, it has been known that HPV can cause benign tumors and warts in humans, such as growing on Human warts common on the skin and mucous membranes near the genitals, condyloma acuminata, and papillomas growing on the mucous membranes. At present, there are more than 80 types of HPV identified, which are divided into skin type HPV and genital tract epithelial HPV according to the epithelial site of infection. About 35 types can infect women's genital tract, and about 20 types are related to tumors. According to the risk of different types of HPV and tumor occurrence, it is divided into low-r...
Claims
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Application Information
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