Method for ultrasound strengthen, extraction and purification of longan and litchi polysaccharide

A lychee polysaccharide and ultrasonic enhancement technology, applied in the field of biochemical engineering, can solve the problems of long extraction time and high extraction temperature

Inactive Publication Date: 2007-10-17
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented method involves an improved process for producing high molecular weight (HMW) algin from seafood waste materials such as Laminaria spp., which has many beneficial health benefits compared with other sources like fish meals. By applying this technique at scale up, we aimed towards achieving higher yields than traditional methods used alone.

Problems solved by technology

This patents describes different ways to improve the quality and quantity of isolated or pure carbochloropolymannuronates (CPMs) found naturally occurring throughout nature during traditional agriculture production systems. These techniques include solvent extractions, centrifugations, acid treatment/degradation, ion exchange resin adhesive binding, solid phase separators like crystal plasma cylinder filters, and supercritical fluid carbon sources called liquefaction liquids containing chilled gels.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 10g of lychee pulp, add water and beat it through a 40-mesh sieve, adjust the liquid / solid ratio to 30:1, and place it in an ultrasonic extraction device with an ultrasonic power of 300W, a duty cycle of 30%, a temperature of 30°C, and an extraction time of 20 minutes. The extract was concentrated in vacuo, precipitated with ethanol, and freeze-dried to obtain 1.6 g of crude polysaccharide.

Embodiment 2

[0021] Take 50g of longan pulp, add water and beat it through a 60-mesh sieve, adjust the liquid / solid ratio to 40:1, place it in a 2.5L circulating ultrasonic extraction device, the ultrasonic power is 1200W, the duty cycle is 30%, the temperature is 40°C, and the extraction time is 30min. The extract was concentrated in vacuo, precipitated with ethanol, and the precipitate was redissolved and purified on a CM-Sephadex column. According to the elution curve of the polysaccharide, the eluate near the absorption peak was collected and dried to obtain 5 g of pure polysaccharide.

Embodiment 3

[0023] Take 500ml of the filtrate after ultrasonic extraction of longan, add 400ppm chitosan at a temperature of 40°C, pH=7, stir at 800rpm for 5 minutes, electric field strength E=20V / cm, stir at 100rpm, electric field action time 30min, polysaccharide The extraction rate is 70%. After ultrasonic extraction of longan, 100ml of ultra-filtered concentrate, at a temperature of 30°C, pH = 6, add 200ppm chitosan and 200ppm polystyrene sodium xanthate, stir at 1000rpm for 5 minutes, electric field strength E = 50V / cm, 100rpm stirring, electric field action time 20min, the polysaccharide extraction rate is 85%.

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Abstract

The invention relates to a novel longan and litchi polysaccharide extraction and purification technology. The longan and litchi polysaccharide is extracted by ultrasonic intensified extraction technology, after liquid-solid separation process, through condensing, flocculating or ethanol settling, filtering, freeze drying, crude polysaccharide can be obtained from the extracting liquid; redissolving concentrated polysaccharide crude extracted liquid or crude polysaccharide, then through fractional precipitation or chromatography purifying, drying and smashing to get longan and litchi polysaccharide purified products. For adopting ultrasonic intensified extraction technology can be used to large scale industrialization production, the extracted temperature is reduced from 90-100 DEGC to 30 DEG C, the extracted time is reduced from 4h to 0.5h, the polysaccharide extraction recovery rate is higher than that of traditional extraction method, the extract of the ultrasonic extraction is more favourable to subsequent separation and purification process to reduce extract purification cost of longan and litchi polysaccharide apparently.

Description

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Claims

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Application Information

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Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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