Method for ultrasound strengthen, extraction and purification of longan and litchi polysaccharide
A lychee polysaccharide and ultrasonic enhancement technology, applied in the field of biochemical engineering, can solve the problems of long extraction time and high extraction temperature
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Embodiment 1
[0019] Take 10g of lychee pulp, add water and beat it through a 40-mesh sieve, adjust the liquid / solid ratio to 30:1, and place it in an ultrasonic extraction device with an ultrasonic power of 300W, a duty cycle of 30%, a temperature of 30°C, and an extraction time of 20 minutes. The extract was concentrated in vacuo, precipitated with ethanol, and freeze-dried to obtain 1.6 g of crude polysaccharide.
Embodiment 2
[0021] Take 50g of longan pulp, add water and beat it through a 60-mesh sieve, adjust the liquid / solid ratio to 40:1, place it in a 2.5L circulating ultrasonic extraction device, the ultrasonic power is 1200W, the duty cycle is 30%, the temperature is 40°C, and the extraction time is 30min. The extract was concentrated in vacuo, precipitated with ethanol, and the precipitate was redissolved and purified on a CM-Sephadex column. According to the elution curve of the polysaccharide, the eluate near the absorption peak was collected and dried to obtain 5 g of pure polysaccharide.
Embodiment 3
[0023] Take 500ml of the filtrate after ultrasonic extraction of longan, add 400ppm chitosan at a temperature of 40°C, pH=7, stir at 800rpm for 5 minutes, electric field strength E=20V / cm, stir at 100rpm, electric field action time 30min, polysaccharide The extraction rate is 70%. After ultrasonic extraction of longan, 100ml of ultra-filtered concentrate, at a temperature of 30°C, pH = 6, add 200ppm chitosan and 200ppm polystyrene sodium xanthate, stir at 1000rpm for 5 minutes, electric field strength E = 50V / cm, 100rpm stirring, electric field action time 20min, the polysaccharide extraction rate is 85%.
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