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Method for preparing glucose by extracting biomass residue using alkaline and acid and enzyme

A technology of biomass residue and alkali extraction, which is applied in the field of enzymatic-acid process, can solve the problems of high operating cost, long hydrolysis cycle, high production cost, etc., and achieve the effect of improving efficiency

Inactive Publication Date: 2007-12-12
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this process, the biomass needs to be pretreated, and the accumulation of oligosaccharides in the intermediate product of the hydrolysis process has a significant inhibitory effect on the catalytic reaction, which affects the yield of degraded glucose. Additional β-glucosidase needs to be added to eliminate the inhibition, resulting in The production cost of the whole process enzyme is high, the hydrolysis cycle is long, and the operation cost is too high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Weigh 0.6g of corncob residue from alkali extraction, put it into a 50ml Erlenmeyer flask, add 5ml of cellulase solution (Trichoderma reesei) with an enzyme activity of 1 FPIU / mL and 15ml of 0.05M citric acid-sodium citrate Buffer (pH 4.5), shake well to keep the pH at 4.5-5.5.

[0027] 2. The first enzymatic hydrolysis: put it in a constant temperature water bath shaker at 50°C for 24 hours, and the oscillation amplitude is 100r / min.

[0028] 3. Centrifuge at 3500rpm for 15 minutes for solid-liquid separation. The supernatant is marked as solution I, removed from the reaction system for later use, and the solid phase residue is marked as residue II.

[0029] 4. The second enzymatic hydrolysis: pour the residue II back into the original Erlenmeyer flask, weigh it, and add 1 to 5 times the amount of 0.05M citric acid-sodium citrate buffer (pH4.5) to keep the pH at 4.5-5.5 , oscillated at 100r / min in a constant temperature water bath shaker at 50°C, and carried out th...

Embodiment 2

[0032] 1. Weigh 0.6g of alkali-extracted corn stalk residue, put it into a 50ml Erlenmeyer flask, and add 5ml of cellulase solution (Aspergillus niger) with an enzyme activity of 1.5FPIU / mL and 15ml of 0.05M citric acid-sodium citrate Buffer (pH 4.5), shake well to keep the pH at 4.5-5.5.

[0033] 2. The first enzymatic hydrolysis: put it in a 50°C constant temperature water bath shaker for 48 hours, and the oscillation amplitude is 100r / min.

[0034] 3. Centrifuge at 3500rpm for 15 minutes for solid-liquid separation. The supernatant is marked as solution I, removed from the reaction system for later use, and the solid phase residue is marked as residue II.

[0035] 4. The second enzymatic hydrolysis: Pour the residue II back into the original Erlenmeyer flask, weigh it, add 1 to 5 times the amount of pH 4.5 0.05M citric acid-sodium citrate buffer, make the pH 4.5-5.5, 50°C Oscillating in a constant temperature water bath oscillator at 100r / min, enzymatic hydrolysis reaction...

Embodiment 3

[0038]1. Weigh 1.0g of birch wood residue extracted by alkali, put it into a 50ml Erlenmeyer flask, and add 5ml of enzyme activity in 1.5FPIU / mL cellulase solution (Trichoderma viride) and 15ml of 0.05M citric acid-sodium citrate Buffer (pH4.5), shake well to keep the pH at 4.5-5.5.

[0039] 2. The first enzymatic hydrolysis: put it in a constant temperature water bath shaker at 50°C for 24 hours, and the oscillation amplitude is 100r / min.

[0040] 3. Centrifuge at 3500rpm for 15 minutes for solid-liquid separation. The supernatant is marked as solution I, removed from the reaction system for later use, and the solid phase residue is marked as residue II.

[0041] 4. The second enzymatic hydrolysis: Pour the residue II back into the original Erlenmeyer flask, weigh it, add 1 to 5 times the amount of 0.05M citric acid-sodium citrate buffer to make the pH 4.5-5.5, and place it at 50°C Oscillate in a constant temperature water bath shaker at 100r / min, enzymatic hydrolysis reacti...

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Abstract

The invention relates to a enzyme- acid method for preparing glucose by using alkaline extracted biomass residual. It takes biomas residual treated with alkaline extraction as raw material, adds biomass residual into pH buffering solution for dissloution and cellulase for enzymolysis; separating solid from liquid after enzymolysis, getting solution I and residual II; adding pH buffering liquid with proper amount according to residual II weight, hydrolyzing by using hydrolyze on residual II, separating solid from liquid and getting solution II; combining solution I and II, adding inorganic acid for hydrolysis, collecting glucose. The invention is characterized in that it can increase conversion rate of cellulose to reductive sugar, the oligosaccharide can be fullly conversed to glucose, which increases glucose productivity.

Description

technical field [0001] The invention relates to the technical field of plant cellulose hydrolysis, in particular to an enzymatic-acid process for preparing glucose from biomass residues extracted by alkali. Background technique [0002] Modern industry has made the development of human civilization equivalent to the progress of the past few thousand years in just one or two hundred years, and at the same time consumed a lot of fossil resources. The development and utilization of renewable energy has become an important strategy for human beings to solve energy and environmental problems . The utilization of water energy, wind energy, solar energy and biomass energy has become a hot topic in today's society, but only biomass is both a renewable energy source and an important carbon resource. [0003] Plants on the earth absorb about 100 billion tons of CO every year 2 , they are emitting a large amount of O 2 At the same time, 150 billion tons of biomass are produced, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02
Inventor 欧阳嘉严婕黄和姜岷许琳严明
Owner NANJING FORESTRY UNIV