Method for preparing glucose by extracting biomass residue using alkaline and acid and enzyme
A biomass residue and alkali extraction technology, which is applied in the field of enzymatic-acid process, can solve the problems of high operating cost, long hydrolysis cycle, and affecting glucose yield, and achieve the effect of improving efficiency
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Embodiment 1
[0026] 1. Weigh 0.6g of corncob residue from alkali extraction, put it into a 50ml Erlenmeyer flask, add 5ml of cellulase solution (Trichoderma reesei) with an enzyme activity of 1FPIU / mL and 15ml of 0.05M citric acid-sodium citrate buffer solution (pH 4.5), shake well to keep the pH at 4.5-5.5.
[0027] 2. The first enzymatic hydrolysis: put it in a constant temperature water bath shaker at 50°C for 24 hours, and the oscillation amplitude is 100r / min.
[0028] 3. Centrifuge at 3500rpm for 15 minutes for solid-liquid separation. The supernatant is marked as solution I, removed from the reaction system for later use, and the solid phase residue is marked as residue II.
[0029] 4. The second enzymatic hydrolysis: pour the residue II back into the original Erlenmeyer flask, weigh it, add 0.05M citric acid-sodium citrate buffer solution (pH 4.5) in 1 to 5 times the amount, make the pH at 4.5-5.5, Shake in a constant temperature water bath shaker at 50°C at 100r / min, carry out th...
Embodiment 2
[0032] 1. Weigh 0.6g of alkali-extracted corn stalk residue, put it into a 50ml Erlenmeyer flask, and add 5ml of cellulase solution (Aspergillus niger) with an enzyme activity of 1.5FPIU / mL and 15ml of 0.05M citric acid-sodium citrate Buffer (pH 4.5), shake well to keep the pH at 4.5-5.5.
[0033] 2. The first enzymatic hydrolysis: put it in a 50°C constant temperature water bath shaker for 48 hours, and the oscillation amplitude is 100r / min.
[0034] 3. Centrifuge at 3500rpm for 15 minutes for solid-liquid separation. The supernatant is marked as solution I, removed from the reaction system for later use, and the solid phase residue is marked as residue II.
[0035] 4. The second enzymatic hydrolysis: Pour the residue II back into the original Erlenmeyer flask, weigh it, add 1 to 5 times the amount of pH 4.5 0.05M citric acid-sodium citrate buffer, make the pH 4.5-5.5, 50°C Oscillating in a constant temperature water bath oscillator at 100r / min, enzymatic hydrolysis reaction...
Embodiment 3
[0038]1. Weigh 1.0g of birch wood residue extracted by alkali, put it into a 50ml Erlenmeyer flask, and add 5ml of enzyme activity in 1.5FPIU / mL cellulase solution (Trichoderma viride) and 15ml of 0.05M citric acid-sodium citrate Buffer (pH 4.5), shake well to keep the pH at 4.5-5.5.
[0039] 2. The first enzymatic hydrolysis: put it in a constant temperature water bath shaker at 50°C for 24 hours, and the oscillation amplitude is 100r / min.
[0040] 3. Centrifuge at 3500rpm for 15 minutes for solid-liquid separation. The supernatant is marked as solution I, removed from the reaction system for later use, and the solid phase residue is marked as residue II.
[0041] 4. The second enzymatic hydrolysis: Pour the residue II back into the original Erlenmeyer flask, weigh it, add 1 to 5 times the amount of 0.05M citric acid-sodium citrate buffer to make the pH 4.5-5.5, and place it at 50°C Oscillate in a constant temperature water bath shaker at 100r / min, enzymatic hydrolysis react...
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