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TEF-1 gene of weight increment per day of pig and application in assistant selecting molecule markers of pigs

A marker-assisted selection, TEF-1 technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Inactive Publication Date: 2007-12-26
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, the research on porcine TEF-1 gene is still blank at home and abroad.

Method used

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  • TEF-1 gene of weight increment per day of pig and application in assistant selecting molecule markers of pigs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1: Detection application of PCR-BshN I-RELP polymorphism in various pig breeds

[0072] Five pig breeds were detected by PCR-BshN I-RFLP: Erhualian (Chinese local pig blood), Tongcheng pig (Chinese local pig blood), Changbai (foreign pig of European blood), Large White (foreign pig of European blood), Du For Locke pigs (exotic pigs of European origin), the gene frequencies of various pig breeds are shown in Table 3, and it was found that the allele G is dominant in several pig breeds.

[0073] Table 3 The pig daily gain TEF-1 gene BshN I polymorphic genotype frequency and gene frequency of clone of the present invention

[0074] Variety

Embodiment 2

[0076] Application examples of association analysis of pig marker traits

[0077] The application of the association analysis between the BshN I-RFLP polymorphism site in the 5' untranslated region of the pig daily gain TEF-1 gene and some carcass and meat quality traits, the genotype detection results showed that there were significant differences in the GG genotype in 159 individuals. 58 individuals, 71 individuals with GC genotype and 30 individuals with CC genotype. In the application of association analysis with some production traits, the analyzed traits include some production traits and meat quality traits. The analyzed traits are the whole daily weight gain . The results are shown in Table 4.

[0078] Table 4 The effect of the TEF-1 gene polymorphism on the daily gain of pigs cloned in the present invention on the whole daily gain

[0079] genotype

[0080] Note: shoulder bet * Expression P** Expressed P<0.01.

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Abstract

This invention relates to cloning and application of pig daily gain-related TEF-1 gene. The cDNA sequence of TEF-1 gene is shown in SEQ ID No.1. There is an A-G mutation at 93bp of SEQ ID No.1, which leads to BshN I-RFLP polymorphism. This invention also discloses a method for preparing pig daily gain-related TEF-1 gene, its primer design method, and the application of TEF-1 gene in molecular marker-assisted selection of pigs.

Description

technical field [0001] The invention belongs to the technical field of animal genetic engineering, and in particular relates to the cloning of pig daily gain TEF-1 gene and its application in pig molecular marker-assisted selection. Background technique [0002] Pork is the main source of animal protein for urban and rural residents in my country. my country's pork production and pig breeding rank first in the world. The development of pig farming has extremely important theoretical and practical significance in my country. Pork has consistently accounted for around 65 percent of total meat production in recent years. Especially with the reform of my country's economic system, farmers' awareness of commodities has gradually increased, and the pig industry is developing rapidly from traditional family sidelines to specialization, scale, intensification, and factoryization. Therefore, it is extremely important to accelerate the development of genetics and breeding to support ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12Q1/68
Inventor 刘榜邢双赵书红朱猛进李奎樊斌余梅李长春
Owner HUAZHONG AGRI UNIV
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