Method and apparatus for inducing artificial hibernation of marine animal
A technology of marine organisms and artificial hibernation, applied in fish farming, application, climate change adaptation, etc., can solve the problems of fish quality decline, high cost of cooling water tank, difficult operation, etc., and achieve the effect of high survival rate
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Embodiment 1
[0064] Example 1: Analysis of Respiration Activity and Intrinsic Biological Rhythm of Flounder
[0065] First, in order to understand artificial hibernation using the internal biological rhythm of marine organisms, it is necessary to analyze the internal biological rhythm possessed by the target fish.
[0066] The experimental conditions for measuring the internal biological rhythms of fish are very demanding. In this experimental condition, at least two or more external environmental conditions must maintain a certain state before it can be identified as an intrinsic biological rhythm (Palmer 1995). Because fish have eyes, seeing light will affect melanin, which will affect the action and physiological activities of fish, making it difficult to observe the internal biological rhythm. In the present invention, a BOD incubator (VS 1203P5N, Vison Co., Seoul, Korea) capable of shielding light and maintaining a specific water temperature (Fig. 4: 19°C, Fig. 6: 21.5°C) was used. ...
Embodiment 2
[0067] Example 2: The average oxygen consumption of flounder according to the decrease of seawater temperature
[0068] According to the analysis method and data of flatfish respiratory activity and internal biological rhythm in the above-mentioned embodiment 1, use the artificial hibernation induction device of the present invention to determine that when the temperature of seawater containing marine organisms gradually decreases, the oxygen consumption of the above-mentioned marine organisms changes significantly Seawater temperature with reduced or no change.
[0069] As a result, as shown in FIG. 8 , the water temperature was lowered from 13° C. to 10° C., and the survival of flatfish was not affected in any way. Therefore, the inventors maintained the seawater temperature at 13°C for 10 minutes, exposed it at 12°C for 20 minutes, and exposed it at 11°C for 30 minutes, and gradually increased the exposure time as the temperature decreased. 120 minutes of exposure time (10 m...
Embodiment 3
[0075] Example 3: Artificial hibernation experiments using live fish and flounder
[0076] Next, the live fish and flounder that have entered artificial hibernation are placed in a box whose internal temperature is kept at 5°C, and the whole surface of the fish is covered with wet Korean paper, and the box is sealed for storage. Recorded the change with storage time, the maintenance time of the live fish and flounder in the above-mentioned box under anhydrous state, and the survival rate of the above-mentioned live fish and flounder, the results are shown in Table 2.
[0077] [Table 2: Results of artificial hibernation experiments using live fish and flounder]
[0078] experiment
[0079] As shown in Table 2, compared with the existing methods, the live fish and flounder that have used the artificial hibernation induction technology of the present invention and the water-free transport packaging technology have a long maintenance time in anhydrous state, and the surv...
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