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System and method for cultivating cells

A technology of cells and substances, applied in the field of systems and methods for culturing cells, can solve problems such as non-uniform stratification of cells, and achieve better effects

Inactive Publication Date: 2008-04-02
ASTRAZENECA AB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the time it takes to pipette into the entire cell suspension (cells and media), the cells have already started to settle to the bottom, thus causing non-uniform stratification of cells within the plant

Method used

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  • System and method for cultivating cells
  • System and method for cultivating cells
  • System and method for cultivating cells

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Experimental program
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Embodiment Construction

[0064] The prior art method illustrated in FIG. 1 has been previously discussed in the "Background Art", which is referred to here without any further discussion.

[0065] 2 and 3, there is schematically illustrated a cell culture system 20 according to at least one embodiment of the present invention. The cell culture system 20 includes a multi-layer cell factory 22, a substance transfer device 42 illustrated here as a funnel 44 connected to a pipe 46, and a holder device 62 for holding the substance transfer device 42.

[0066] The multi-layer cell factory 22 illustrated here has ten layers 24a to 24j, but other numbers of layers are also conceivable. Each layer 24a to 24j has two opposing relatively wide plate-shaped rectangular wall sides 25 and four relatively narrow strip-shaped wall sides 26 interconnected by the edge portions of the opposing rectangular wall sides. Defined fluid receiving volume. Rectangular geometric shapes are only examples, and other geometric shapes ar...

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PUM

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Abstract

The invention relates to a method of introducing a substance into a multi-layered cell factory. A substance is poured into a substance transferring device e.g. a funnel and a tube connected to the cell factory, which directly transfers the substance into the cell factory arranged with its layers extending substantially vertically. The invention also relates to a cell-cultivation system.

Description

Technical field [0001] The present invention relates to a method of introducing substances into a multi-layer cell factory. The present invention also relates to a cell culture system including a multi-layer cell factory. Background technique [0002] Multi-layer cell factories are widely used as part of cell work in laboratory. Cell factories are available from different suppliers, such as Nunc A / S, Roskilde, Denmark and Corning Incorporated Life Sciences, Acton, MA 01720, USA. Cell factories of different sizes with different layers and suitable for different purposes can be obtained. For example, the 10-stack (ten-layer) format of these cell factories was found to be particularly useful because a large number of cells can be produced with relatively little extra work, equipment, or space. However, the current standard methods for filling these cell factories have some problems, which lead to high time and labor costs and / or lead to unsatisfactory filling and harvesting, which m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/04C12M1/18C12N5/07C12N5/071
CPCC12M23/34C12M23/04Y10T137/8593
Inventor L·桑德A·孙德伯格
Owner ASTRAZENECA AB