Method for extracting saponin from oat bran
A technology of oat bran and extraction method, which is applied in the direction of chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problem of separation of refractory saponins and other compounds, achieve good extraction effect, high economic benefit, low cost effect
Inactive Publication Date: 2012-05-30
TIANJIN UNIV OF SCI & TECH
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Problems solved by technology
Difficulty separating saponins from other compounds due to chemical similarities between the above compounds and saponins
Method used
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[0033] The technical content of the present invention will be further described below in conjunction with embodiments; the following embodiments are illustrative and not restrictive, and the scope of protection of the present invention cannot be limited by the following embodiments.
[0034] Specific examples are:
[0035] (1). Weigh 1 kg of oat bran, add 4L of methanol, extract for 1 hour at 50°C, repeat the extraction 4 times, combine the filtrate, and recover the solvent to obtain a methanol extract.
[0036] (2). For the extract, add distilled water 20 times the mass of the extract (mL:g) and fully disperse to obtain an aqueous dispersion of methanol extract. Add 4 times the volume of the methanol extract of n-butanol (mL:g). Fully extract, collect the n-butanol extract, repeat the extraction with n-butanol 3 times, combine the extracts, and recover the n-butanol to obtain the n-butanol extract.
[0037] (3) The n-butanol extract is treated with 4 times the amount of ethyl acetate...
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The present invention relates to a method to extract saponin from oat bran, wherein the oat bran is extracted with methanol as the extraction solvent, the extracted liquid is collected, and centrifuged, then the supernatant is condensed and dried to obtain the extract, which is adequately dispersed with distilled water and extracted with n-butanol, then the extracted liquid with the solvent thereof recovered is dried, then the dried product is treated with ethyl acetate, the solvent is volatized, and then the crude extract of oat bran is obtained. Macroporous absorbant resin on the crude extract is rinsed off successively with 50 percent (v / v) and 70 percent (v / v) ethanol-water solution, the liquid rinsed with 70 percent (v / v) ethanol-water solution is collected, condensed and dried, and then the saponin of oat bran is obtained. The present invention effectively overcome the difficulty on separation of saponin in oat bran from other compounds, and oat bran with low cost is adopted as the raw material, multiple extractions are adopted with ethanol-water solution, the extraction effect is good with high economic benefits; the crude product is separated with macroporous absorbent resin, condensed and dried to obtain the saponin of oat bran with high purity.
Description
Technical field [0001] The invention relates to a method for extracting saponin, in particular to a method for extracting saponin from oat bran. Background technique [0002] Saponins are a relatively complex type of glycoside compounds. They are called saponins because their aqueous solutions produce soap-like long-lasting foam when shaken. Saponins are composed of saponins and sugars, uronic acid or other organic acids. Common sugars in saponins are glucose, galactose, rhamnose, arabinose, xylose and other pentoses. Common uronic acids are Glucuronic acid, galacturonic acid, etc. These sugars or uronic acids are first combined into sugar chains and then connected to sapogenins. The extraction method of saponins is mainly determined according to the nature and solubility of saponins. Because the saponins molecules contain more sugar groups and are highly polar, saponins are generally soluble in water, dilute alcohol, hot ethanol and hot methanol, and almost insoluble in ether. ...
Claims
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IPC IPC(8): C07H1/08
Inventor 张民陈海霞魏红裴颖
Owner TIANJIN UNIV OF SCI & TECH