Preparation of kilogram-grade scale high-purity monosialotetrahexosylganglioside

Abstract

The invention discloses a manufacturing technique for extracting, separating and purifying kilogram-grade scale high purity GM1 using fresh pig brain tissue as raw material, which is characterized in that large sized extraction pot and adsorption resin with large holes, DEAE Sephadex A-25 and silica gel layer are utilized to build a set of manufacturing technique. The invention has the advantagesof elimination of toxic solvent chloroform in mobile phase in traditional method, less pollution for whole process, simple process, low cost and applicability to industrialized production.

Description

Technical field [0001] The invention relates to a preparation method of kilogram-scale high-purity monosialotetrahexosyl ganglioside (GM1), belonging to the field of biomedicine. Background technique [0002] Gangliosides (GLS) are a class of acidic glycolipids containing sialic acid, and are important components of mammalian cell membranes, with the highest content in brain tissue. The composition of gangliosides is very complex. Different gangliosides contain different numbers of sialic acids and connection methods of sugar groups. G can be used to represent gangliosides, and M, D, and T can represent single, di, and tri Sialic acid group, the subscript numbers 1, 2, and 3 represent tetraglycosyl, triosyl, and diglycosylceramide respectively, and GM1 represents a ganglioside composed of a single sialic acid group and tetraglycosylceramide. . Studies have shown that gangliosides with significant biological functions are GM1 connected with a single sialic acid, which can p...

AI Technical Summary

Problems solved by technology

However, there are many types of gangliosides, and their content in brain tissue is small, especially their molecular composition, structure, physical and chemical properties and other factors are very similar, so the extraction and separation of high-purity GM1 is difficult, especially in large-scale industrial extraction processes. No reports and applications

So far, most domestic research on GM1 extraction and separation is still at the laboratory scale and level, and has not been effectively applied. The techniques used mainly include the Momoi.T method and the method of using microbial transformation, but the cost is too high to be large-scale clinical application

There is no batch production method and process for single-component GLS such as GM1 in China. Most of the existing separation and purification methods have disadvantages such as cumbersome operation steps, complicated process, long cycle, and not suitable for mass production.

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